IgG3 antibody | LO-MG3-7
Biotin labeled Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7 (MCA423B) used for antibody class evaluation by ELISA.
Image caption:
Anti-IGF1R and anti-HER2/neu antibodies elicited by cell vaccines co-targeting HER2/neu and IGF1R.
Analysis of antibody isotypes induced by cell vaccines as in Figure 3A (n = 3 mice per group).
From: De Giovanni C, Landuzzi L, Palladini A, Ianzano ML, Nicoletti G, Ruzzi F, Amici A, Croci S, Nanni P, Lollini PL.
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 2019 Apr 11;11(4). pii: E517.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse IgG3 Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG3-7
- Isotype
- IgM
- Specificity
- IgG3
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7 recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG. |
- Target Species
- Mouse
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Polyclonal and monoclonal mouse IgG3.
- Approx. Protein Concentrations
- IgM concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as a coating antibody |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rat IgM:Biotin | 302008 | C E IB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Goat anti Rat IgM:Biotin | ||||||
| Goat anti Rat IgM:RPE | 302009 | F | 0.5 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Goat anti Rat IgM:RPE | ||||||
| Goat anti Rat IgM:HRP | STAR116P | E WB | 0.5 mg | Log in | |||
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| Description | Goat anti Rat IgM:HRP | ||||||
References for IgG3 antibody
-
Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
Prot Biol Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-8. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16. -
Zivkovic, I. et al. (2012) Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.
Innate Immun. 18: 333-42. -
DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 11 (4)Apr 11 [Epub ahead of print].
- RRID
- AB_321849
- UniProt
- P03987
- Entrez Gene
- AI324046
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
MCA423
166112
180613
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