IgG3 antibody | LO-MG3-7

Rat anti Mouse IgG3 Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG3-7
Isotype
IgM
Specificity
IgG3
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA423
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 mg loader
List Price Your Price
loader
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Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7 recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG.

Target Species
Mouse
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Polyclonal and monoclonal mouse IgG3.
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml as a coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG3 antibody

  1. Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
    Prot Biol Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-8.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J Immunol Methods. 66 (2): 261-9.
  3. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J Immunol Methods. 71 (1): 9-16.
  4. Zivkovic, I. et al. (2012) Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.
    Innate Immun. 18: 333-42.
  5. DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
    Cancers (Basel). 11 (4)Apr 11 [Epub ahead of print].

RRID
AB_321849
UniProt
P03987
Entrez Gene
AI324046
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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