IgG3 antibody | LO-MG3-7

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Rat anti Mouse IgG3 Heavy Chain:Biotin

Rat anti Mouse IgG3 Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG3-7
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA423B E 0.5 mg
MCA423 E 0.25 mg
Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7 recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG.

Product Details

Target Species
Mouse
Product Form
Purified IgM conjugated to Biotin - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Polyclonal and monoclonal mouse IgG3.
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Approx. Protein Concentrations
IgM concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P03987 Related reagents
Entrez Gene
AI324046 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
Regulatory
For research purposes only

Applications of IgG3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml 1.0ug/ml
ELISA 5ug/ml as a coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a secondary reagent in a sandwich ELISA together with MCA1292 as the capture reagent and and Streptavidin:HRP (STAR5B) as the detection reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Product Specific References

References for IgG3 antibody

  1. Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
    Prot. Biol. Fluids. Peters Ed: 29th Colloquim (1981). Pergamon Press, Oxford and N.Y., pp 615-618.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J. Immunol. Methods. 66(2): 261-269.
  3. Bazin H, et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J. Immunol. Methods. 71: 9-16.
  4. Zivkovic, I. et al. (2012) Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.
    Innate Immun. 18: 333-42.