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CD90 antibody | F7D5

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Anti Mouse CD90 Antibody, clone F7D5 thumbnail image 1 Anti Mouse CD90 Antibody, clone F7D5 thumbnail image 2 Anti Mouse CD90 Antibody, clone F7D5 thumbnail image 3
Anti Mouse CD90 Antibody, clone F7D5 gallery image 1
Figure A. RPE conjugated rat anti mouse CD4 (MCA2691PE). Figure B. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A488 conjugated mouse anti mouse CD90 (MCA02A488). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5™ cell analyzer.
Anti Mouse CD90 Antibody, clone F7D5 gallery image 2
Figure A. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A647 conjugated mouse IgM isotype control (MCA722A647). Figure B. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A647 conjugated mouse anti mouse CD90 (MCA02A647). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5™ cell analyzer.
Anti Mouse CD90 Antibody, clone F7D5 gallery image 3
Published customer image:
Mouse anti Mouse CD90 antibody, clone F7D5 (MCA02R) used for T cell depletion of bone marrow and subsequent evaluation of chimerism by flow cytometry.
Image caption:
Donor chimerism and alloreactivity. a Percentage donor T cell chimerism in peripheral blood lymphocytes, determined by Thy1.1 (recipient) and Thy1.2 (donor) positivity. b percentage alloreactive CD3+Vβ6+ T cells. Results are mean ± SEM from 5 animals in BMT+SPL+SRI and BMT only group and from 4 animals in BMT+SPL+vehicle group. Statistically significant differences are depicted with * (one-tailed p<0.05). NS = not significant

From: Gobin V, Van Steendam K, Fevery S, Tilleman K, Billiau AD, Denys D, Deforce DL. Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression. J Neuroimmune Pharmacol. 2013 Sep;8(4):934-43.

Mouse anti Mouse CD90:Alexa Fluor® 488

Mouse anti Mouse CD90:Alexa Fluor® 647

Mouse anti Mouse CD90:Pacific Blue®

Mouse anti Mouse CD90

Mouse anti Mouse CD90:RPE

Product Type
Monoclonal Antibody
Clone
F7D5
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA02A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA02A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA02PB
Datasheet
F
SDS
100 Tests/1ml loader

MCA02RT
Datasheet
CT F
SDS
25 µg loader

MCA02R
Datasheet
CT F
SDS
0.25 mg loader

MCA02PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.

The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979).

Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.

Product Details

Target Species
Mouse
Product Form
IgM fraction conjugated to Alexa Fluor 488 - liquid
Product Form
IgM fraction conjugated to Alexa Fluor 647 - liquid
Product Form
IgM fraction conjugated to Pacific Blue - liquid
Product Form
IgM fraction - liquid
Product Form
IgM fraction conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
IgM fraction prepared by ammonium sulfate precipitation
Preparation
IgM fraction prepared by ammonium sulfate precipitation
Preparation
IgM fraction prepared by ammonium sulfate precipitation
Preparation
IgM fraction prepared by ammonium sulphate precipitation
Preparation
IgM fraction prepared by ammonium sulfate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Approx. Protein Concentrations
Ig concentration 0.05 mg/ml
Approx. Protein Concentrations
Ig concentration 0.05 mg/ml
Approx. Protein Concentrations
IgM concentration 0.05 mg/ml
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01831
Entrez Gene
Thy1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0030336 negative regulation of cell migration
GO:0030425 dendrite
GO:0030426 growth cone
GO:0031362 anchored to external side of plasma membrane
GO:0034235 GPI anchor binding
GO:0043547 positive regulation of GTPase activity
GO:0045121 membrane raft
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Cytotoxic Assays
Flow Cytometry 1/10 1/25
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD90 antibody

  1. Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
    Eur J Immunol. 9 (11): 875-86.
  2. Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.
    Int Immunol. 14 (12): 1439-47.
  3. DeVries-vanDerZwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.
    Blood. 89 (7): 2596-601.
  4. Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.
    J Neurosci. 31: 6481-92.
  5. Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.
    Exp Eye Res. 127: 206-14.
  6. Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.
    PLoS One. 10: e0117615.
  7. Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.
    Diabetes. 37: 204-8.
  8. Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.
    Blood. 102: 740-8.
  9. Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.
    EMBO J. 18: 2702-6.
  10. Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses.
    Blood. 97 (11): 3498-504.
  11. Billiau, A.D. et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.
    Blood. 100 (5): 1894-902.
  12. Ishikawa, N. et al. (1998) Early cytokine responses during intestinal parasitic infections.
    Immunology. 93 (2): 257-63.
  13. Ishigaki, H. et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
    Microbiol Immunol. 50 (8): 655-62.
  14. Logan, G.J. et al. (2004) CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection.
    Immunol Cell Biol. 82 (2): 132-5.
  15. Gobin, V. et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.
    J Neuroimmune Pharmacol. 8 (4): 934-43.
  16. van Pel, M. et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.
    Bone Marrow Transplant. 32 (1): 15-22.
  17. Oosterwegel, M.A. et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.
    J Immunol. 163 (5): 2634-9.
  18. Vadivelu, S. et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.
    Stem Cells Transl Med. pii: sctm.2014-0107.
  19. Wang, Y.L. et al. (2015) Electrospun and woven silk fibroin/poly(lactic-co-glycolic acid) nerve guidance conduits for repairing peripheral nerve injury.
    Neural Regen Res. 10 (10): 1635-42.
  20. Liu, X. et al. (2017) Thy-1 interaction with Fas in lipid rafts regulates fibroblast apoptosis and lung injury resolution.
    Lab Invest. (3): 256-67.
  21. Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
    Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print]
  22. Bernard-Marissal, N. et al. (2015) Dysfunction in endoplasmic reticulum-mitochondria crosstalk underlies SIGMAR1 loss of function mediated motor neuron degeneration.
    Brain. 138 (Pt 4): 875-90.
  23. Takahama, S. et al. (2017) Retinal Astrocytes and GABAergic Wide-Field Amacrine Cells Express PDGFRα: Connection to Retinal Ganglion Cell Neuroprotection by PDGF-AA.
    Invest Ophthalmol Vis Sci. 58 (11): 4703-11.
  24. Zhu, B. et al. (2019) GAIN domain-mediated cleavage is required for activation of G protein-coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist.
    J Biol Chem. pii: jbc.RA119.008234. Oct 18 [Epub ahead of print].
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