CD90 antibody | F7D5
Mouse anti Mouse CD90
- Product Type
- Monoclonal Antibody
- Clone
- F7D5
- Isotype
- IgM
- Specificity
- CD90
Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB. The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979). Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes. |
- Target Species
- Mouse
- Product Form
- IgM fraction - liquid.
- Preparation
- IgM fraction prepared by ammonium sulphate precipitation from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
0.09% sodium azide. - Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml.
- Fusion Partners
- Spleen cells from immunized AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Cytotoxic Assays | |||
Flow Cytometry | 1/10 | 1/25 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | ||||||
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) |
References for CD90 antibody
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Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
Eur J Immunol. 9 (11): 875-86. -
Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.
Diabetes. 37: 204-8. -
DeVries-vanDerZwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.
Blood. 89 (7): 2596-601. -
Ishikawa, N. et al. (1998) Early cytokine responses during intestinal parasitic infections.
Immunology. 93 (2): 257-63. -
Oosterwegel, M.A. et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.
J Immunol. 163 (5): 2634-9. -
Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.
EMBO J. 18: 2702-6. -
Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses.
Blood. 97 (11): 3498-504. -
Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.
Int Immunol. 14 (12): 1439-47.
View The Latest Product References
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Billiau, A.D. et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.
Blood. 100 (5): 1894-902. -
van Pel, M. et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.
Bone Marrow Transplant. 32 (1): 15-22. -
Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.
Blood. 102: 740-8. -
Ishigaki, H. et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
Microbiol Immunol. 50 (8): 655-62. -
Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.
J Neurosci. 31: 6481-92. -
Gobin, V. et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.
J Neuroimmune Pharmacol. 8 (4): 934-43. -
Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.
Exp Eye Res. 127: 206-14. -
Vadivelu, S. et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.
Stem Cells Transl Med. pii: sctm.2014-0107. -
Wang, Y.L. et al. (2015) Electrospun and woven silk fibroin/poly(lactic-co-glycolic acid) nerve guidance conduits for repairing peripheral nerve injury.
Neural Regen Res. 10 (10): 1635-42. -
Bernard-Marissal, N. et al. (2015) Dysfunction in endoplasmic reticulum-mitochondria crosstalk underlies SIGMAR1 loss of function mediated motor neuron degeneration.
Brain. 138 (Pt 4): 875-90. -
Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.
PLoS One. 10: e0117615. -
Liu, X. et al. (2017) Thy-1 interaction with Fas in lipid rafts regulates fibroblast apoptosis and lung injury resolution.
Lab Invest. (3): 256-67. -
Naaldijk, Y. et al. (2017) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
Neuropathol Appl Neurobiol. 43 (4): 299-314. -
Takahama, S. et al. (2017) Retinal Astrocytes and GABAergic Wide-Field Amacrine Cells Express PDGFRα: Connection to Retinal Ganglion Cell Neuroprotection by PDGF-AA.
Invest Ophthalmol Vis Sci. 58 (11): 4703-11. -
Zhu, B. et al. (2019) GAIN domain-mediated cleavage is required for activation of G protein-coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist.
J Biol Chem. pii: jbc.RA119.008234. Oct 18 [Epub ahead of print]. -
Bürger, S. et al. (2020) Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons.
Int J Mol Sci. 22 (1): 369. -
Qiu, A.W. et al. (2021) IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy.
Cell Death Dis. 12 (11): 1057. -
Xing, J. et al. (2023) Post-injury born oligodendrocytes incorporate into the glial scar and contribute to the inhibition of axon regeneration.
Development. 150 (8): dev201311. -
Ma, R. et al. (2023) RGC-Net: An Automatic Reconstruction and Quantification Algorithm for Retinal Ganglion Cells Based on Deep Learning.
Transl Vis Sci Technol. 12 (5): 7. -
Xing, J. et al. (2023) Experimental upregulation of developmentally downregulated ribosomal protein large subunits 7 and 7A promotes axon regeneration after injury in vivo.
Exp Neurol. 368: 114510. -
Llorente, I.L. et al. (2021) Patient-derived glial enriched progenitors repair functional deficits due to white matter stroke and vascular dementia in rodents.
Sci Transl Med. 13 (590) [Epub ahead of print]. -
Lukomska, A. et al. (2024) Upregulation of developmentally-downregulated miR-1247-5p promotes neuroprotection and axon regeneration in vivo.
Neurosci Lett. 823: 137662. -
Sun, S. et al. (2020) Opa1 Deficiency Leads to Diminished Mitochondrial Bioenergetics With Compensatory Increased Mitochondrial Motility.
Invest Ophthalmol Vis Sci. 61 (6): 42. -
Zwanzig, A. et al. (2021) Neuroprotective effects of glial mediators in interactions between retinal neurons and Müller cells.
Exp Eye Res. 209: 108689. -
Qiu, A.W. et al. (2021) IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy.
Cell Death Dis. 12 (11): 1057.
- Synonyms
- THY1
- RRID
- AB_323481
- UniProt
- P01831
- Entrez Gene
- Thy1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005100 Rho GTPase activator activity
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0030336 negative regulation of cell migration
- GO:0030425 dendrite
- GO:0030426 growth cone
- GO:0031362 anchored to external side of plasma membrane
- View More GO Terms
- GO:0034235 GPI anchor binding
- GO:0043547 positive regulation of GTPase activity
- GO:0045121 membrane raft
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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