CD90 antibody | F7D5
Mouse anti Mouse CD90 antibody, clone F7D5 (MCA02R) used for T cell depletion of bone marrow and subsequent evaluation of chimerism by flow cytometry.
Image caption:
Donor chimerism and alloreactivity. a Percentage donor T cell chimerism in peripheral blood lymphocytes, determined by Thy1.1 (recipient) and Thy1.2 (donor) positivity. b percentage alloreactive CD3+Vβ6+ T cells. Results are mean ± SEM from 5 animals in BMT+SPL+SRI and BMT only group and from 4 animals in BMT+SPL+vehicle group. Statistically significant differences are depicted with * (one-tailed p<0.05). NS = not significant
From: Gobin V, Van Steendam K, Fevery S, Tilleman K, Billiau AD, Denys D, Deforce DL. Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression. J Neuroimmune Pharmacol. 2013 Sep;8(4):934-43.
Mouse anti Mouse CD90:Alexa Fluor® 488
Mouse anti Mouse CD90:Alexa Fluor® 647
Mouse anti Mouse CD90:Pacific Blue®
Mouse anti Mouse CD90
Mouse anti Mouse CD90:RPE
- Product Type
- Monoclonal Antibody
- Clone
- F7D5
- Isotype
- IgM
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA02A488 | F | 100 Tests/1ml |
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| MCA02A647 | F | 100 Tests/1ml |
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| MCA02PB | F | 100 Tests/1ml |
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| MCA02R | CT F | 0.25 mg |
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| MCA02RT | CT F | 25 µg |
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| MCA02PE | F | 100 Tests |
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Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.
The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979).
Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979).
Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
Product Details
- Target Species
- Mouse
- Product Form
- IgM fraction conjugated to Alexa Fluor 488 - liquid
- Product Form
- IgM fraction conjugated to Alexa Fluor 647 - liquid
- Product Form
- IgM fraction conjugated to Pacific Blue - liquid
- Product Form
- IgM fraction - liquid
- Product Form
- IgM fraction conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- IgM fraction prepared by ammonium sulfate precipitation
- Preparation
- IgM fraction prepared by ammonium sulfate precipitation
- Preparation
- IgM fraction prepared by ammonium sulfate precipitation
- Preparation
- IgM fraction prepared by ammonium sulphate precipitation
- Preparation
- IgM fraction prepared by ammonium sulfate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Approx. Protein Concentrations
- Ig concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Ig concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgM concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01831
- Entrez Gene
- Thy1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005100 Rho GTPase activator activity
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0030336 negative regulation of cell migration
- GO:0030425 dendrite
- GO:0030426 growth cone
- GO:0031362 anchored to external side of plasma membrane
- GO:0034235 GPI anchor binding
- GO:0043547 positive regulation of GTPase activity
- GO:0045121 membrane raft
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD90 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Cytotoxic Assays | |||
| Flow Cytometry | 1/10 | 1/25 | |
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
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| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
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| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for CD90 antibody
-
Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
Eur J Immunol. 9 (11): 875-86. -
Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.
Int Immunol. 14 (12): 1439-47. -
DeVries-vanDerZwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.
Blood. 89 (7): 2596-601. -
Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.
J Neurosci. 31: 6481-92. -
Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.
Exp Eye Res. 127: 206-14. -
Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.
PLoS One. 10: e0117615. -
Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.
Diabetes. 37: 204-8. -
Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.
Blood. 102: 740-8. -
Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.
EMBO J. 18: 2702-6. -
Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses.
Blood. 97 (11): 3498-504. -
Billiau, A.D. et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.
Blood. 100 (5): 1894-902. -
Ishikawa, N. et al. (1998) Early cytokine responses during intestinal parasitic infections.
Immunology. 93 (2): 257-63. -
Ishigaki, H. et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
Microbiol Immunol. 50 (8): 655-62. -
Logan, G.J. et al. (2004) CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection.
Immunol Cell Biol. 82 (2): 132-5. -
Gobin, V. et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.
J Neuroimmune Pharmacol. 8 (4): 934-43. -
van Pel, M. et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.
Bone Marrow Transplant. 32 (1): 15-22. -
Oosterwegel, M.A. et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.
J Immunol. 163 (5): 2634-9. -
Vadivelu, S. et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.
Stem Cells Transl Med. pii: sctm.2014-0107. -
Wang, Y.L. et al. (2015) Electrospun and woven silk fibroin/poly(lactic-co-glycolic acid) nerve guidance conduits for repairing peripheral nerve injury.
Neural Regen Res. 10 (10): 1635-42. -
Liu, X. et al. (2017) Thy-1 interaction with Fas in lipid rafts regulates fibroblast apoptosis and lung injury resolution.
Lab Invest. (3): 256-67. -
Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print] -
Bernard-Marissal, N. et al. (2015) Dysfunction in endoplasmic reticulum-mitochondria crosstalk underlies SIGMAR1 loss of function mediated motor neuron degeneration.
Brain. 138 (Pt 4): 875-90. -
Takahama, S. et al. (2017) Retinal Astrocytes and GABAergic Wide-Field Amacrine Cells Express PDGFRα: Connection to Retinal Ganglion Cell Neuroprotection by PDGF-AA.
Invest Ophthalmol Vis Sci. 58 (11): 4703-11. -
Zhu, B. et al. (2019) GAIN domain-mediated cleavage is required for activation of G protein-coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist.
J Biol Chem. pii: jbc.RA119.008234. Oct 18 [Epub ahead of print]. -
Bürger, S. et al. (2020) Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons.
Int J Mol Sci. 22 (1): 369. -
Qiu, A.W. et al. (2021) IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy.
Cell Death Dis. 12 (11): 1057. -
Xing, J. et al. (2021) Post-injury born oligodendrocytes integrate into the glial scar and inhibit growth of regenerating axons by premature myelination
boRxiv. Oct 20 [Epub ahead of print].
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