CD90 antibody | F7D5

Mouse anti Mouse CD90

Product Type
Monoclonal Antibody
Clone
F7D5
Isotype
IgM
Specificity
CD90

Product Code Applications Pack Size List Price Your Price Qty
MCA02R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
CT F 0.25 mg loader
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loader
MCA02RT
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SDS Safety Datasheet SDS
CT F 25 µg loader
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loader
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Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.

The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake et al. 1979).

Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.

Target Species
Mouse
Product Form
IgM fraction - liquid
Preparation
IgM fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Cytotoxic Assays
Flow Cytometry 1/10 1/25
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD90 antibody

  1. Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
    Eur J Immunol. 9 (11): 875-86.
  2. Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.
    Int Immunol. 14 (12): 1439-47.
  3. DeVries-vanDerZwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.
    Blood. 89 (7): 2596-601.
  4. Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.
    J Neurosci. 31: 6481-92.
  5. Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.
    Exp Eye Res. 127: 206-14.
  6. Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.
    PLoS One. 10: e0117615.
  7. Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.
    Diabetes. 37: 204-8.
  8. Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.
    Blood. 102: 740-8.
  9. View The Latest Product References
  10. Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.
    EMBO J. 18: 2702-6.
  11. Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses.
    Blood. 97 (11): 3498-504.
  12. Billiau, A.D. et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.
    Blood. 100 (5): 1894-902.
  13. Ishikawa, N. et al. (1998) Early cytokine responses during intestinal parasitic infections.
    Immunology. 93 (2): 257-63.
  14. Ishigaki, H. et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
    Microbiol Immunol. 50 (8): 655-62.
  15. Logan, G.J. et al. (2004) CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection.
    Immunol Cell Biol. 82 (2): 132-5.
  16. Gobin, V. et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.
    J Neuroimmune Pharmacol. 8 (4): 934-43.
  17. van Pel, M. et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.
    Bone Marrow Transplant. 32 (1): 15-22.
  18. Oosterwegel, M.A. et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.
    J Immunol. 163 (5): 2634-9.
  19. Vadivelu, S. et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.
    Stem Cells Transl Med. pii: sctm.2014-0107.
  20. Wang, Y.L. et al. (2015) Electrospun and woven silk fibroin/poly(lactic-co-glycolic acid) nerve guidance conduits for repairing peripheral nerve injury.
    Neural Regen Res. 10 (10): 1635-42.
  21. Liu, X. et al. (2017) Thy-1 interaction with Fas in lipid rafts regulates fibroblast apoptosis and lung injury resolution.
    Lab Invest. (3): 256-67.
  22. Naaldijk, Y. et al. (2016) Effect of systemic transplantation of bone marrow-derived mesenchymal stem cells on neuropathology markers in APP/PS1 Alzheimer mice.
    Neuropathol Appl Neurobiol. Feb 26. [Epub ahead of print]
  23. Bernard-Marissal, N. et al. (2015) Dysfunction in endoplasmic reticulum-mitochondria crosstalk underlies SIGMAR1 loss of function mediated motor neuron degeneration.
    Brain. 138 (Pt 4): 875-90.
  24. Takahama, S. et al. (2017) Retinal Astrocytes and GABAergic Wide-Field Amacrine Cells Express PDGFRα: Connection to Retinal Ganglion Cell Neuroprotection by PDGF-AA.
    Invest Ophthalmol Vis Sci. 58 (11): 4703-11.
  25. Zhu, B. et al. (2019) GAIN domain-mediated cleavage is required for activation of G protein-coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist.
    J Biol Chem. pii: jbc.RA119.008234. Oct 18 [Epub ahead of print].
  26. Bürger, S. et al. (2020) Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons.
    Int J Mol Sci. 22 (1): 369.
  27. Qiu, A.W. et al. (2021) IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy.
    Cell Death Dis. 12 (11): 1057.
  28. Xing, J. et al. (2021) Post-injury born oligodendrocytes integrate into the glial scar and inhibit growth of regenerating axons by premature myelination
    boRxiv. Oct 20 [Epub ahead of print].

Synonyms
THY1
RRID
AB_323481
UniProt
P01831
Entrez Gene
Thy1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0030336 negative regulation of cell migration
GO:0030425 dendrite
GO:0030426 growth cone
GO:0031362 anchored to external side of plasma membrane
GO:0034235 GPI anchor binding
GO:0043547 positive regulation of GTPase activity
GO:0045121 membrane raft
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
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