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CD80 antibody | RM80

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Rat anti Mouse CD80:RPE

Product Type
Monoclonal Antibody
Clone
RM80
Isotype
IgG2a
Specificity
CD80

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Product Code Applications Pack Size List Price Your Price Qty
MCA2462PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse CD80 antibody, clone RM80 recognizes mouse CD80 (B7-1), a ~60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.

CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Immunogen
BCL1 cells expressing CD80.
Fusion Partners
Spleen cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
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Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
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Description Mouse Seroblock FcR

Source Reference

  1. Nuriya, S. et al. (1996) The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity.
    Int Immunol. 8 (6): 917-26.

References for CD80 antibody

  1. Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
    Int Immunol. 9 (5): 637-44.
  2. Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
    J Immunol. 167 (9): 4981-6.
  3. Jin LP et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
    J Immunol. 173 (6): 3612-9.
  4. Inada, T. et al. (2009) Vaccines using dendritic cells, differentiated with propofol, enhance antitumor immunity in mice.
    Immunopharmacol Immunotoxicol. 31 (1): 150-7.
  5. Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
    J Clin Invest. 119 (12): 3723-38.
  6. Deng, J. et al. (2010) Dendritic cells loaded with ultrasound-ablated tumour induce in vivo specific antitumour immune responses.
    Ultrasound Med Biol. 36 (3): 441-8.
  7. Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
    J Immunol. 187: 4517-29.
  8. He, Y.T. et al. (2016) In vitro generation of cytotoxic T lymphocyte response using dendritic cell immunotherapy in osteosarcoma.
    Oncol Lett. 12 (2): 1101-6.
    9. View The Latest Product References
  9. Kim, I. et al. (2016) Immunological characterization of de novo and recall alloantibody suppression by CTLA4Ig in a mouse model of allosensitization.
    Transpl Immunol. 38: 84-92.
  10. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.

Synonyms
B7-1
RRID
AB_999255
UniProt
Q00609
Entrez Gene
Cd80
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
GO:0046641 positive regulation of alpha-beta T cell proliferation

MCA2462PE

157949 166563

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