CD8 antibody | YTS169.4

Rat anti Mouse CD8:FITC

Product Type
Monoclonal Antibody
Clone
YTS169.4
Isotype
IgG2b
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1768F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD8 antibody, clone YTS169.4 recognizes the murine CD8 cell surface antigen, expressed by a subset of T lymphocytes.

Rat anti Mouse CD8 antibody, clone YTS169.4 exhibits depleting activity when used in vivo.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD8 antibody

  1. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
    Eur J Immunol. 20 (12): 2747-55.
  2. Bemelman, F. et al. (1998) Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose.
    J Immunol. 160 (6): 2645-8.
  3. Cobbold SP et al. (1984) Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo.
    Nature. 312 (5994): 548-51.
  4. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
    J Immunol. 161 (11): 5813-6.
  5. Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
    J Immunol. 162 (1): 486-93.
  6. Scotland, R.S. et al. (2011) Sex-differences in resident immune cell phenotype underlies more efficient acute inflammatory responses in female mice.
    Blood. 118: 5918-27.
  7. Matsubara, K. et al. (2016) Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice.
    Hypertens Res. 39 (6): 407-14.
  8. Jaffar, Z. et al. (2002) A key role for prostaglandin I2 in limiting lung mucosal Th2, but not Th1, responses to inhaled allergen.
    J Immunol. 169 (10): 5997-6004.
  9. View The Latest Product References
  10. Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.
    Mol Ther. 20 (4): 808-19.
  11. Abd-elhakim, Y.M. et al. (2016) Hemato-immunologic impact of subchronic exposure to melamine and/or formaldehyde in mice.
    J Immunotoxicol. 13 (5): 713-22.
  12. Ismail, S.A.A. (2017) Ameliorative Potential of Spirulina platensis against Lead Acetate Induced Immuno-Suppression and Kidney Apoptosis in Rats
    Ann Clin Pathol 5(5): 1120.
  13. Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
    Sci Rep. 10 (1): 15157.
  14. de Souza, T.A. et al. (2018) Relationship between the inflammatory tumor microenvironment and different histologic types of canine mammary tumors.
    Res Vet Sci. 119: 209-14.
  15. Lejeune, P. et al. (2021) Immunostimulatory effects of targeted thorium-227 conjugates as single agent and in combination with anti-PD-L1 therapy.
    J Immunother Cancer. (10):e002387.

UniProt
P01731
P10300
Entrez Gene
Cd8a
Cd8b1
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0005515 protein binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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