CD8 antibody | YTS169.4

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/5

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Mouse Seroblock FcR	BUF041A	F	0.1 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Mouse Seroblock FcR
Mouse Seroblock FcR	BUF041B	F	0.5 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Mouse Seroblock FcR

References for CD8 antibody

1. Cobbold, S.P. et al. (1984) Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo.
   Nature. 312 (5994): 548-51.
2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
   Eur J Immunol. 20 (12): 2747-55.
3. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
   J Immunol. 161 (11): 5813-6.
4. Bemelman, F. et al. (1998) Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose.
   J Immunol. 160 (6): 2645-8.
5. Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
   J Immunol. 162 (1): 486-93.
6. Jaffar, Z. et al. (2002) A key role for prostaglandin I2 in limiting lung mucosal Th2, but not Th1, responses to inhaled allergen.
   J Immunol. 169 (10): 5997-6004.
7. Scotland, R.S. et al. (2011) Sex-differences in resident immune cell phenotype underlies more efficient acute inflammatory responses in female mice.
   Blood. 118: 5918-27.
8. Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.
   Mol Ther. 20 (4): 808-19.
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9. Abd-elhakim, Y.M. et al. (2016) Hemato-immunologic impact of subchronic exposure to melamine and/or formaldehyde in mice.
   J Immunotoxicol. 13 (5): 713-22.
10. Matsubara, K. et al. (2016) Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice.
    Hypertens Res. 39 (6): 407-14.
11. de Souza, T.A. et al. (2018) Relationship between the inflammatory tumor microenvironment and different histologic types of canine mammary tumors.
    Res Vet Sci. 119: 209-14.
12. Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
    Sci Rep. 10 (1): 15157.
13. Lejeune, P. et al. (2021) Immunostimulatory effects of targeted thorium-227 conjugates as single agent and in combination with anti-PD-L1 therapy.
    J Immunother Cancer. (10):e002387.

UniProt

P01731

P10300

Entrez Gene

Cd8a

Cd8b1

GO Terms

GO:0002456 T cell mediated immunity

GO:0016021 integral to membrane

GO:0007166 cell surface receptor linked signaling pathway

GO:0009897 external side of plasma membrane

GO:0042803 protein homodimerization activity

GO:0045065 cytotoxic T cell differentiation

GO:0050850 positive regulation of calcium-mediated signaling

GO:0005515 protein binding

MCA1768A647

1807

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