CD40 antibody | 3/23

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MCA1143PE
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Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.

Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader
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Description Rat IgG2a Negative Control:RPE

References for CD40 antibody

  1. Parry, S.L. et al. (1994) Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes.
    Eur J Immunol. 24 (4): 974-9.
  2. Hasbold, J. et al. (1994) Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies.
    Eur J Immunol. 24 (8): 1835-42.
  3. Hasbold, J. & Klaus, G.G. (1994) B cells from CBA/N mice do not proliferate following ligation of CD40.
    Eur J Immunol. 24 (1): 152-7.
  4. Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
    J Clin Invest. 119 (12): 3723-38.
  5. Mohan, J. et al. (2005) Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure.
    Immunology. 116: 122-33.
  6. Mukhopadhyay, S. et al. (2004) Activation of murine macrophages by Neisseria meningitidis and IFN-gamma in vitro: distinct roles of class A scavenger and Toll-like pattern recognition receptors in selective modulation of surface phenotype.
    J Leukoc Biol. 76: 577-84.
  7. Russo, S. et al. (2003) Platelet-activating factor mediates CD40-dependent angiogenesis and endothelial-smooth muscle cell interaction.
    J Immunol. 171: 5489-97.
  8. Shakib, S. et al. (2009) Checkpoints in the development of thymic cortical epithelial cells.
    J Immunol. 182: 130-7.
  9. View The Latest Product References
  10. Ogasawara, K. et al. (2002) Profiles of cell-to-cell interaction of Mycobacterium intracellulare-induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission.
    Clin Exp Immunol. 129: 272-80.
  11. Salomon, B. et al. (1998) Three populations of mouse lymph node dendritic cells with different origins and dynamics.
    J Immunol. 160: 708-17.
  12. Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
    Int Immunol. 12: 1669-75.
  13. Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
    J Immunol. 187: 4517-29.
  14. Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
    Eur J Immunol. 45 (2): 480-91.
  15. Malada-Edelstein, Y.F. et al. (2017) Regulatory role of cytosolic phospholipase A2 alpha in the induction of CD40 in microglia.
    J Neuroinflammation. 14 (1): 33.

Flow Cytometry

Functional Assays

RRID
AB_321691
UniProt
P27512
Entrez Gene
Cd40

MCA1143PE

149012

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