CD4/CD8 | YTS191.1 / KT15

Rat anti Mouse CD4:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
YTS191.1 / KT15
Isotype
Cocktail
Specificity
CD4/CD8

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DC034
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Rat anti Mouse CD4:FITC/CD8:RPE dual colour reagent was developed for the simultaneous recognition of mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognises murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognises a non-polymorphic epitope on the murine CD8 alpha chain.

Target Species
Mouse
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2b (RAT)
RPE reagent: IgG2a (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

References for CD4/CD8

  1. Darby, C.R. et al. (1992) Evidence that long-term cardiac allograft survival induced by anti-CD4 monoclonal antibody does not require depletion of CD4+ T cells.
    Transplantation. 54 (3): 483-90.
  2. Lis, M. et al. (2013) Modulatory effects of bestatin on T and B lymphocyte subsets and the concentration of cytokines released by Th1/Th2 lymphocytes in cyclophosphamide-treated mice
    Centr Eur J Immunol 38: 42-53
  3. Ding, J. et al. (2012) Immune responses to a recombinant attenuated Salmonella typhimurium strain expressing a Taenia solium oncosphere antigen TSOL18.
    Comp Immunol Microbiol Infect Dis. 36 (1): 17-23.
  4. Suszko, A. & Obmińska-Mrukowicz, B. (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.
    J Ethnopharmacol. 145 (1): 109-17.
  5. Aravind, S. et al. (2015) Protective effects of recombinant glycoprotein D based prime boost approach against duck enteritis virus in mice model.
    Microb Pathog. 88: 78-86.
  6. Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.
    J Immunotoxicol. 12 (4): 322-9.
  7. Szczypka, M. et al. (2020) Selegiline and clomipramine effects on lymphocyte subsets, regulatory T cells and sheep red blood cell (SRBC)-induced humoral immune response after in vivo administration in mice.
    Eur J Pharmacol. 887: 173560.
  8. Piekarska, J. et al. (2020) Effect of aqueous extract from Scutellaria baicalensis Georgi roots on CD4+ and CD8+ T cell responses during experimental infection with Trichinella spiralis in mice.
    Pol J Vet Sci. 23 (4): 501-10.
  9. View The Latest Product References
  10. Piekarska, J. et al. (2022) Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (Lonicera caerulea L.) in the Course of Experimental Trichinellosis in Mice.
    Molecules. 27 (6): 1949.

RRID
AB_323752
UniProt
P06332
P01731
P10300
Entrez Gene
Cd8a
Cd4
Cd8b1
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus
GO:0005515 protein binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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