CD4/CD8 | YTS191.1/KT15

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Rat anti Mouse CD4:FITC/CD8:RPE

Product Type
Dual Color Reagent
Clone
YTS191.1/KT15
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC034 F 100 Tests
Rat anti Mouse CD4:FITC/CD8:RPE dual colour reagent was developed for the simultaneous recognition of mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognises murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognises a non-polymorphic epitope on the murine CD8 alpha chain.

Product Details

Target Species
Mouse
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 1 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2b (RAT)
RPE reagent: IgG2a (RAT)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P06332 Related reagents
P01731 Related reagents
P10300 Related reagents
Entrez Gene
Cd8a Related reagents
Cd4 Related reagents
Cd8b1 Related reagents
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus
GO:0005515 protein binding
Regulatory
For research purposes only

Applications of CD4/CD8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Product Specific References

References for CD4/CD8

  1. Darby, C.R. et al. (1992) Evidence that long-term cardiac allograft survival induced by anti-CD4 monoclonal antibody does not require depletion of CD4+ T cells.
    Transplantation. 54 (3): 483-90.
  2. Lis, M. et al. (2013) Modulatory effects of bestatin on T and B lymphocyte subsets and the concentration of cytokines released by Th1/Th2 lymphocytes in cyclophosphamide-treated mice
    Centr Eur J Immunol 38: 42-53
  3. Ding, J. et al. (2012) Immune responses to a recombinant attenuated Salmonella typhimurium strain expressing a Taenia solium oncosphere antigen TSOL18.
    Comp Immunol Microbiol Infect Dis. 36 (1): 17-23.
  4. Suszko A & Obmi&324;ska-Mrukowicz B (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.
    J Ethnopharmacol. 145 (1): 109-17.
  5. Aravind, S. et al. (2015) Protective effects of recombinant glycoprotein D based prime boost approach against duck enteritis virus in mice model.
    Microb Pathog. 88: 78-86.
  6. Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.
    J Immunotoxicol. 12 (4): 322-9.