Rat anti Mouse CD4:FITC/CD8:RPE dual colour reagent was developed for the simultaneous recognition of mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognises murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognises a non-polymorphic epitope on the murine CD8 alpha chain.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 1 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG2b (RAT)
RPE reagent: IgG2a (RAT)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
T cell mediated immunity
integral to membrane
cell surface receptor linked signaling pathway
external side of plasma membrane
protein homodimerization activity
cytotoxic T cell differentiation
positive regulation of calcium-mediated signaling
endoplasmic reticulum lumen
endoplasmic reticulum membrane
T cell selection
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of T cell activation
response to protein stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for CD4/CD8
Darby, C.R. et al. (1992) Evidence that long-term cardiac allograft survival induced by anti-CD4 monoclonal antibody does not require depletion of CD4+ T cells.
Transplantation. 54 (3): 483-90.
Lis, M. et al. (2013) Modulatory effects of bestatin on T and B lymphocyte subsets and the concentration of cytokines released by Th1/Th2 lymphocytes in cyclophosphamide-treated mice
Centr Eur J Immunol 38: 42-53
Ding, J. et al. (2012) Immune responses to a recombinant attenuated Salmonella typhimurium strain expressing a Taenia solium oncosphere antigen TSOL18.
Comp Immunol Microbiol Infect Dis. 36 (1): 17-23.
Suszko, A. & Obmińska-Mrukowicz, B. (2013) Influence of polysaccharide fractions isolated from Caltha palustris L. on the cellular immune response in collagen-induced arthritis (CIA) in mice. A comparison with methotrexate.
J Ethnopharmacol. 145 (1): 109-17.
Aravind, S. et al. (2015) Protective effects of recombinant glycoprotein D based prime boost approach against duck enteritis virus in mice model.
Microb Pathog. 88: 78-86.
Zimecki, M. et al. (2015) Immune function in cyclophosphamide-treated mice is restored by the T-cell-tropic isoxazole derivative R-13.
J Immunotoxicol. 12 (4): 322-9.
Szczypka, M. et al. (2020) Selegiline and clomipramine effects on lymphocyte subsets, regulatory T cells and sheep red blood cell (SRBC)-induced humoral immune response after in vivo administration in mice.
Eur J Pharmacol. 887: 173560.
Piekarska, J. et al. (2020) Effect of aqueous extract from Scutellaria baicalensis Georgi roots on CD4+ and CD8+ T cell responses during experimental infection with Trichinella spiralis in mice.
Pol J Vet Sci. 23 (4): 501-10.
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