CD200R antibody | OX-110
Rat anti Mouse CD200R:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-110
- Isotype
- IgG2a
- Specificity
- CD200R
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD200R antibody, clone OX-110 recognizes mouse CD200R, a cell surface glycoprotein (also known as OX2R. Mouse CD200R is a 326 amino acid, ~48 kDa single pass type I transmembrane glycoprotein, expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells (Wright et al.2003). CD200-CD200R interaction may be involved in the control of myeloid cellular function (Minas and Liversidge 2006). Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Fusion protein mCD200RCD4d3+4
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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References for CD200R antibody
-
Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
J Immunol. 171 (6): 3034-46. -
Zhao, J. et al. (2009) Evasion by stealth: inefficient immune activation underlies poor T cell response and severe disease in SARS-CoV-infected mice.
PLoS Pathog. 5 (10): e1000636. -
Björck, P. et al. (2011) Plasmacytoid Dendritic Cell Dichotomy: Identification of IFN-{alpha} Producing Cells as a Phenotypically and Functionally Distinct Subset.
J Immunol. 186: 1477-85. -
Dellacasa-Lindberg, I. et al. (2011) Migratory Activation of Primary Cortical Microglia upon Infection with Toxoplasma gondii.
Infect Immun. 79: 3046-52. -
Krejsek, J. et al. (2010) Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation.
Perfusion 25: 389-97. -
Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
Mol Immunol. 45: 1126-35. -
Costello, D.A. et al. (2011) Long term potentiation is impaired in membrane glycoprotein CD200-deficient mice: a role for Toll-like receptor activation.
J Biol Chem. 286 (40): 34722-32. -
Simelyte, E. et al. (2008) CD200-Fc, a novel antiarthritic biologic agent that targets proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis.
Arthritis Rheum. 58: 1038-43.
View The Latest Product References
-
Whitmore, L.C. et al. (2014) NOX2 protects against progressive lung injury and multiple organ dysfunction syndrome.
Am J Physiol Lung Cell Mol Physiol. 307: L71-82. -
Chitnis, T. et al. (2007) Elevated neuronal expression of CD200 protects Wlds mice from inflammation-mediated neurodegeneration.
Am J Pathol. 170: 1695-712. -
Larson, D. et al. (2012) Chronic helminth infection reduces basophil responsiveness in an IL-10-dependent manner.
J Immunol. 188: 4188-99. -
Torrero, M.N. et al. (2009) CD200R surface expression as a marker of murine basophil activation.
Clin Exp Allergy. 39 (3): 361-9. -
Hernangómez M et al. (2012) CD200-CD200R1 interaction contributes to neuroprotective effects of anandamide on experimentally induced inflammation.
Glia 60 (9): 1437-50. -
Bain, C.C. & Mowat, A.M. (2012) CD200 receptor and macrophage function in the intestine.
Immunobiology 217 (6): 643-51. -
Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
Int Immunopharmacol. 11 (7): 794-801. -
Akkaya, M. et al. (2013) Dissection of agonistic and blocking effects of CD200 receptor antibodies.
PLoS One 8 (5): e63325. -
Cassard L et al. (2012) Fcγ receptors inhibit mouse and human basophil activation.
J Immunol. 189 (6): 2995-3006. -
Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
J Immunol. Jul 6. pii: 1600052. [Epub ahead of print] -
Toya, E.E. & Ohba, M. (2015) Improved Long-term Culture of Epidermal Stem Cells Utilizing CD200R-expressing Feeder Cells
Showa Uni J Med Sci 27 (2) 83-91. -
Lago, N. et al. (2018) CD200 modulates spinal cord injury neuroinflammation and outcome through CD200R1.
Brain Behav Immun. 73: 416-26. -
Qian, H. et al. (2023) Activation of the CD200/CD200R1 axis attenuates neuroinflammation and improves postoperative cognitive dysfunction via the PI3K/Akt/NF-κB signaling pathway in aged mice.
Inflamm Res. 72 (12): 2127-44.
- Synonyms
- OX2R
- RRID
- AB_566626
- UniProt
- Q9ES57
- Entrez Gene
- Cd200r1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0009897 external side of plasma membrane
MCA2281PE
166582
1702
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