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CD200 antibody | OX-90

Rat anti Mouse CD200:RPE

Product Type
Monoclonal Antibody
Clone
OX-90
Isotype
IgG2a
Specificity
CD200

Product Code Applications Pack Size List Price Your Price Qty
MCA1958PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2.

CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.

The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Mouse CD200-rat CD4 fusion protein.
Fusion Partners
Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
List Price Your Price
Description Rat IgG2a Negative Control:RPE

References for CD200 antibody

  1. Hoek, R.M. et al. (2000) Down-regulation of the macrophage lineage through interaction with OX2 (CD200).
    Science. 290 (5497): 1768-71.
  2. Nathan, C. & Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200?
    Nat Immunol. 2 (1): 17-9.
  3. Rijkers, E.S. (2007) Ligation of CD200R by CD200 is not required for normal murine myelopoiesis.
    Eur J Haematol. 79: 410-6.
  4. Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
    Mol Immunol. 45: 1126-35.
  5. Burger, P.E. et al. (2009) High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
    Stem Cells. 27: 2220-8.
  6. Ko, Y.C. et al (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.
  7. Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
    J Neuropathol Exp Neurol. 68: 159-67.
  8. Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
    Int Immunopharmacol. 11 (7): 794-801.
  9. View The Latest Product References
  10. Garza, L.A. et al. (2011) Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.
    J Clin Invest. 121: 613-22.
  11. Montiel, M. et al. (2015) Melatonin decreases brain apoptosis, oxidative stress, and CD200 expression and increased survival rate in mice infected by Venezuelan equine encephalitis virus.
    Antivir Chem Chemother. 24 (3-4): 99-108.
  12. Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
    J Immunol. 197 (4): 1489-97.
  13. Liu, C. et al. (2018) The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation.
    J Inflamm (Lond). 15: 28.
  14. Lago, N. et al. (2018) CD200 modulates spinal cord injury neuroinflammation and outcome through CD200R1.
    Brain Behav Immun. 73: 416-26.
  15. Tonecka, K. et al. (2021) The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
    Int J Mol Sci. 22 (10): 5358.
  16. Pannunzio, B. et al. (2022) CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
    Cells. 11 (11): 1786.

Flow Cytometry

Immunohistology - Frozen

Synonyms
OX2
RRID
AB_324640
UniProt
O54901
Entrez Gene
Cd200
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane

MCA1958PE

158103

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