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MMAE antibody | AbD50348

Human anti MMAE

Product Type
Monoclonal Antibody
Clone
AbD50348
Isotype
Fab antibody
Specificity
MMAE

Product Code Applications Pack Size List Price Your Price Qty
New TZA057
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-MMAE Antibody, clone AbD50348 recognizes the Monomethyl Auristatin E (MMAE) toxin, an antimitotic agent which inhibits cell division by blocking the polymerization of tubulin. Because of its toxicity, MMAE is not used as a drug itself, but is rather linked to monoclonal antibodies to form an antibody-drug conjugate through a cathepsin-cleavable linker that is stable in the extracellular fluid. Upon binding into the target cell, the linker is cleaved by cathepsin, realeasing the toxin in the cell and activating the antimitotic mechanism.

This antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour.

View a summary of SpyCatcher products

Human anti-MMAE Antibody, clone AbD50348 has been shown to recognize the MMAE toxin in both free and antibody-conjugated forms. It has also been shown to cross-react with the MMAE closely related toxin MMAF and MMAD. It does not recognize unbound antibody drugs. Clone AbD50348 can therefore be used to measure the level of several MMAE bound drug in serum from patients.
20th Anniversary

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Monomethyl Auristatin E (MMAE)
Affinity
The monovalent intrinsic affinity of AbD50348ad was measured as KD = 5 nM by real time, label free molecular interaction analysis on immobilized brentuximab vedotin.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD50348ad can be used as the detection antibody for MMAE toxin in its free and also in its coupled format eg. brentuximab vedotin, in a PK ELISA together with TZA056 as the capture antibody.

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Brentuximab Vedotin HCA349 E 0.1 mg loader
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loader
Description Human anti Brentuximab Vedotin
Human anti Brentuximab Vedotin HCA350 E 0.1 mg loader
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loader
Description Human anti Brentuximab Vedotin
Human anti Brentuximab Vedotin HCA351 E 0.1 mg loader
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Description Human anti Brentuximab Vedotin
Human anti Brentuximab Vedotin HCA352 E 0.1 mg loader
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Description Human anti Brentuximab Vedotin
Human anti CD30 (Brentuximab Biosimilar) MCA6149 E 0.2 mg loader
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loader
Description Human anti CD30 (Brentuximab Biosimilar)
Human anti MMAE TZA056 E 0.1 mg
List Price Your Price
Description Human anti MMAE
Human anti MMAE:HRP TZA057P E 0.1 mg
List Price Your Price
Description Human anti MMAE:HRP

TZA057

167540

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with MMAE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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