VEGF antibody | CD19-4G11

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Mouse anti Human VEGF:Biotin

Product Type
Monoclonal Antibody
Clone
CD19-4G11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6287B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human VEGF antibody, clone CD19-4G11, recognizes vascular endothelial growth factor alpha (VEGF-A) which forms a homodimer and is a potent stimulator of angiogenesis of both normal and cancerous cells. It acts as a regulator of vasculogenesis, angiogenesis and endothelial cell growth. VEGF-A is secreted by many different cell types such as endothelial cells (Nissen et al. 1998), smooth muscle cells (Brogi et al. 1994), neutrophils (Gaudry et al. 1997), platelets (Banks et al. 1998), macrophages and 60% of all tumors (Berse et al. 1992).

The biotinylated Mouse anti Human VEGF antibody, clone CD19-4G11 (MCA6287B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human VEGF antibody, clone CD17-1E1 (MCA6286GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. Coli derived recombinant full length human VEGF (Ala27-Arg147)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q969D9
Entrez Gene
TSLP
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of VEGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human VEGF MCA6286GA E 0.1 mg loader

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