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VAV1 antibody | 1582CT802.383

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Anti VAV1 Antibody, clone 1582CT802.383 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti VAV1 Antibody, clone 1582CT802.383 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti VAV1 Antibody, clone 1582CT802.383 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with VAV1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to VAV1 (molecular weight 95 kDa).
Anti VAV1 Antibody, clone 1582CT802.383 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of VAV1 from Raji whole cell lysate. The Input lane is 10% input; the VAV1-IP lane is the immunoprecipitation with the VAV1 antibody (2.5 µg, VMA00494); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 μg, MCA928). Western blot analysis was performed using VAV1 antibody (1/1,000, VMA00494) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to VAV1.

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WB IP

Mouse anti VAV1

Product Type
PrecisionAb Monoclonal
Clone
1582CT802.383
Isotype
IgG1
Format
Purified
Specificity
VAV1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00494
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human VAV1 antibody recognizes VAV1.

VAV1 is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for VAV1 (provided by RefSeq, Apr 2012).

Mouse anti Human VAV1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti VAV1 detects a band of approximately 95 kDa in Raji cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human VAV1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P15498
Entrez Gene
VAV1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0046872 metal ion binding
GO:0007264 small GTPase mediated signal transduction
GO:0008624 induction of apoptosis by extracellular signals
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0035023 regulation of Rho protein signal transduction
GO:0048011 nerve growth factor receptor signaling pathway

VMA00494

160712

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