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STAT3 (pSer727) antibody | 23G5

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Anti STAT3 (pSer727) Antibody, clone 23G5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti STAT3 (pSer727) Antibody, clone 23G5 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti STAT3 (pSer727) Antibody, clone 23G5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti STAT3 antibody (VMA00242) or (B) Mouse anti STAT3 (pSer727) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 52 second or a (B) 300 second exposure. STAT3 (pSer727) has a molecular weight of 84 kDa.
Anti STAT3 (pSer727) Antibody, clone 23G5 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of STAT3 (pSer727) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity form each phosphprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

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WB

Mouse anti STAT3 (pSer727)

Product Type
PrecisionAb Monoclonal
Clone
23G5
Isotype
IgG1
Format
Purified
Specificity
STAT3 (pSer727)

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Product Code Applications Pack Size List Price Your Price Qty
VMA00755
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti STAT3 antibody recognizes signal transducer and activator of transcription 3, also known as DNA-binding protein APRF, acute-phase response factor and signal transducer and activator of transcription 3, when phosphorylated at serine 727. STAT3 is a member of the STAT family of transcription factors, which play a key role in numerous processes including cell differentiation, proliferation and transformation. Serine 727 is a key STAT3 phosphorylation site. Ser727 is phosphorylated by multiple kinases including mitogen-activated kinases (MAPK), mammalian target of rapamycin kinase (mTOR), cyclin-dependent kinase 5 (CDK5) and nemo-like kinase (Wakahara et al. 2012). There is an ongoing debate in the scientific literature around the impact of the phosphorylation at serine 727 (Wakahara et al. 2012). Some reports indicate that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727 (Yokogami et al. 2000). Other reports show STAT3 activation independent of serine 727 (Wakahara et al. 2012). The role of serine 727 is likely dependent on the larger cellular context. PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti STAT3 (pSer727) detects a band of approximately 84 kDa in HepG2 + EGF (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to KLH
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
List Price Your Price
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P40763
Entrez Gene
STAT3
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006928 cellular component movement
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0032355 response to estradiol stimulus
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0044419 interspecies interaction between organisms
GO:0046983 protein dimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0070102 interleukin-6-mediated signaling pathway

VMA00755

180704

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