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Siglec-10 antibody | 5G6

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Anti Human Siglec-10 Antibody, clone 5G6 thumbnail image 1
Anti Human Siglec-10 Antibody, clone 5G6 gallery image 1
Siglec-10 tranfected CHO cells stained with Mouse anti Human siglec-10 (MCA5783GA) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B).

Mouse anti Human Siglec-10:RPE

Product Type
Monoclonal Antibody
Clone
5G6
Isotype
IgG1
Specificity
Siglec-10

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Product Code Applications Pack Size List Price Your Price Qty
MCA5783PE
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Mouse anti Human Siglec-10 antibody, clone 5G6 recognizes human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, (Kivi et al. 2009). This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Mouse anti Human Siglec-10 antibody, clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9 (Munday et al. 2001).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Recombinant human Siglec-10, fused with the Fc region of human IgG
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for Siglec-10 antibody

  1. Munday, J. et al. (2001) Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.
    Biochem J. 355 (Pt 2): 489-97.
  2. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
    Proc Natl Acad Sci U S A. 103 (20): 7765-70.
  3. Kivi, E. et al. (2009) Human Siglec-10 can bind to vascular adhesion protein-1 and serves as its substrate.
    Blood. 114 (26): 5385-92.
  4. Ju, B. et al. (2022) Elevated CD19+Siglec-10+ B cell levels are correlated with systemic lupus erythematosus disease activity.
    Int Immunopharmacol. 102: 108403.

Further Reading

  1. Crocker, P.R. (2005) Siglecs in innate immunity.
    Curr Opin Pharmacol. 5 (4): 431-7.

Synonyms
Sialic ACID-BINDING IG-LIKE Lectin 10
UniProt
Q96LC7
Entrez Gene
SIGLEC10
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding

MCA5783PE

156708 163089 INN0911R

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