Browsing Modes for Business Users

SET antibody | AB02/4F6

ISO 9001 Quality Assurance Manufacturing | Print
IP-MS Validated
Anti SET Antibody, clone AB02/4F6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti SET Antibody, clone AB02/4F6 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti SET Antibody, clone AB02/4F6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with SET antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to SET (molecular weight 41 kDa).
Anti SET Antibody, clone AB02/4F6 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of SET from K562 whole cell lysate. The Input lane is 10% input; the SET-IP lane is the immunoprecipitation with the SET antibody (2.5 µg, VMA00620); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA691). Western blot analysis was performed using SET antibody (1/1,000, VMA00620) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to SET.

Filter by Application:

WB IP

Mouse anti SET

Product Type
PrecisionAb Monoclonal
Clone
AB02/4F6
Isotype
IgG2b
Format
Purified
Specificity
SET

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00620
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
List Price Your Price
loader

Mouse anti Human SET antibody recognizes SET, also known as 2PP2A, IGAAD, TAF-I or SET nuclear oncogene.

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Oct 2011).

Mouse anti Human SET antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti SET antibody detects a band of approximately 41 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human SET (aa 1-290)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q01105
Entrez Gene
SET
GO Terms
GO:0006260 DNA replication
GO:0006334 nucleosome assembly
GO:0042393 histone binding
GO:0005829 cytosol
GO:0004864 protein phosphatase inhibitor activity
GO:0005783 endoplasmic reticulum
GO:0005654 nucleoplasm
GO:0006337 nucleosome disassembly
GO:0006913 nucleocytoplasmic transport
GO:0008601 protein phosphatase type 2A regulator activity
GO:0016071 mRNA metabolic process
GO:0016564 transcription repressor activity
GO:0035067 negative regulation of histone acetylation
GO:0043234 protein complex
GO:0043524 negative regulation of neuron apoptosis
GO:0048471 perinuclear region of cytoplasm

VMA00620

170517

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note