SET antibody | AB02/4F6

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Mouse anti SET

SET antibody recognizes SET, also known as 2PP2A, IGAAD, TAF-I or SET nuclear oncogene.

SET Antibody with Control Lysate

SET antibody recognizes SET, also known as 2PP2A, IGAAD, TAF-I or SET nuclear oncogene.

Product Type
PrecisionAb™ Monoclonal
Clone
AB02/4F6
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00620 WB 100 µl
VMA00620KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SET antibody recognizes SET, also known as 2PP2A, IGAAD, TAF-I or SET nuclear oncogene.

The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Oct 2011).

Mouse anti Human SET antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti SET antibody detects a band of approximately 41 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human SET (aa 1-290)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q01105 Related reagents
Entrez Gene
SET Related reagents
GO Terms
GO:0006260 DNA replication
GO:0006334 nucleosome assembly
GO:0042393 histone binding
GO:0005829 cytosol
GO:0004864 protein phosphatase inhibitor activity
GO:0005783 endoplasmic reticulum
GO:0005654 nucleoplasm
GO:0006337 nucleosome disassembly
GO:0006913 nucleocytoplasmic transport
GO:0008601 protein phosphatase type 2A regulator activity
GO:0016071 mRNA metabolic process
GO:0016564 transcription repressor activity
GO:0035067 negative regulation of histone acetylation
GO:0043234 protein complex
GO:0043524 negative regulation of neuron apoptosis
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SET antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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