RUVBL1 antibody | AB04/2E9

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Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RUVBL1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to RUVBL1 (molecular weight 50 kDa)

Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RUVBL1 (1 & 2) or a scrambled siRNA (-) probed with (A) Mouse anti RUVBL1 antibody (**VMA00529**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 10 second and (B) 60 second exposure.

Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of RUVBL1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RUVBL1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti RUVBL1

Product Type: PrecisionAb Monoclonal
Clone: AB04/2E9
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00529 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human RUVBL1 antibody recognizes RuvB-like 1 protein, also known as 49 kDa TATA box-binding protein-interacting protein, ECP-54, INO80 complex subunit H, NMP 238 or pontin 52.

The RUVBL1 gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants (provided by RefSeq, Jan 2016)

Mouse anti Human RuvB-like 1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti RUVBL1 detects a band of approximately 50 kDa in K562 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli-derived recombinant human RUVBL1 (amino acids 1 - 456)

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q9Y265
Entrez Gene: RUVBL1
GO Terms: GO:0005515 protein binding; GO:0003678 DNA helicase activity; GO:0016020 membrane; GO:0005524 ATP binding; GO:0005794 Golgi apparatus; GO:0005815 microtubule organizing center; GO:0006310 DNA recombination; GO:0006350 transcription; GO:0006357 regulation of transcription from RNA polymerase II promoter; GO:0007067 mitosis; GO:0007283 spermatogenesis; GO:0016363 nuclear matrix; GO:0034080 CenH3-containing nucleosome assembly at centromere; GO:0035267 NuA4 histone acetyltransferase complex; GO:0040008 regulation of growth; GO:0043967 histone H4 acetylation; GO:0043968 histone H2A acetylation; GO:0051301 cell division; GO:0071339 MLL1 complex
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of RUVBL1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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