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RUVBL1 antibody | AB04/2E9

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siRNA Validated
Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with RUVBL1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to RUVBL1 (molecular weight 50 kDa)
Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RUVBL1 (1 & 2) or a scrambled siRNA (-) probed with (A) Mouse anti RUVBL1 antibody (VMA00529) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 10 second and (B) 60 second exposure.
Anti RUVBL1 Antibody, clone AB04/2E9 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of RUVBL1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RUVBL1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti RUVBL1

Product Type
PrecisionAb Monoclonal
Clone
AB04/2E9
Isotype
IgG2b
Format
Purified
Specificity
RUVBL1

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00529
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human RUVBL1 antibody recognizes RuvB-like 1 protein, also known as 49 kDa TATA box-binding protein-interacting protein, ECP-54, INO80 complex subunit H, NMP 238 or pontin 52.

The RUVBL1 gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants (provided by RefSeq, Jan 2016)

Mouse anti Human RuvB-like 1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RUVBL1 detects a band of approximately 50 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human RUVBL1 (amino acids 1 - 456)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q9Y265
Entrez Gene
RUVBL1
GO Terms
GO:0005515 protein binding
GO:0003678 DNA helicase activity
GO:0016020 membrane
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005815 microtubule organizing center
GO:0006310 DNA recombination
GO:0006350 transcription
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007067 mitosis
GO:0007283 spermatogenesis
GO:0016363 nuclear matrix
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0035267 NuA4 histone acetyltransferase complex
GO:0040008 regulation of growth
GO:0043967 histone H4 acetylation
GO:0043968 histone H2A acetylation
GO:0051301 cell division
GO:0071339 MLL1 complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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