RPS6KA3 antibody

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Mouse anti RPS6KA3

Mouse anti-human RPS6KA3 antibody recognizes ribosomal protein S6 kinase alpha-3, also known as MAP kinase-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, RSK-2, S6K-alpha-3, insulin-stimulated protein kinase 1, or p90-RSK 3.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00459 WB 100 µl
Mouse anti Human RPS6KA3 antibody recognizes the ribosomal protein S6 kinase alpha-3, also known as MAP kinase-activated protein kinase 1b, insulin-stimulated protein kinase 1, p90-RSK 3 or ribosomal S6 kinase 2.

RPS6KA3 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in RPS6KA3 gene have been associated with Coffin-Lowry syndrome (CLS) (provided by RefSeq, Jul 2008), additionally mutations in the RPS6KA3 gene have been associated with a non-syndromic mental retardation X-linked-19 (MRX19).

Mouse anti Human RPS6KA3 antibody detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPS6KA3 detects a band of approximately 83 kDa in A431 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody isolated from ascites by saturated ammonium sulfate precipitation followed by dialysis against PBS
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified His-tagged fragment of human RPS6KA3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P51812 Related reagents
Entrez Gene
RPS6KA3 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0001501 skeletal system development
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0007417 central nervous system development
GO:0008063 Toll signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RPS6KA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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