RPS5 antibody | rCD02-2C12

Mouse anti RPS5

Product Type
PrecisionAb Monoclonal
Clone
rCD02-2C12
Isotype
IgG2b
Format
Purified
Specificity
RPS5

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VMA00903
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Mouse anti RPS5 antibody recognizes 40S ribosomal protein S5 (RPS5).

Ribosomes are complexes that translate mRNA into protein, and consist of a small 40S subunit and a large 60S subunit (Ameismeier et al. 2018). RPS5 is a component of the 40S subunit of ribosomes (Visweswaraiah and Hinnebusch 2017). RPS5 has been found to regulate P13K/Akt, NF-kappaB and MAP kinase (MAPK) pathways in osteoclastogenesis, and has been suggested as a potential target for treatment of osteoporosis (Chen et al. 2017). Many mammalian ribosomal proteins, including RPS5, appear to also have non-mitochondrial roles. RPS5 has been found to enter the nucleus and nucleoli in mouse cells (Matragkou et al. 2009).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti RPS5 antibody detects a band of approximately 23 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli--derived recombinant protein of amino acids 1-204 of human RPS5
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
As supplied, 12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P46782
Entrez Gene
RPS5
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0003729 mRNA binding
GO:0003735 structural constituent of ribosome
GO:0006413 translational initiation
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0006450 regulation of translational fidelity
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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