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Rala antibody | AB01/3E3

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siRNA Validated
Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with RALA antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to RALA (molecular weight 24 kDa)
Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RALA (1 & 2) or a scrambled siRNA (-) probed with Mouse anti RALA antibody (VMA00772) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 244 second (chemiluminescent channel) and 30 second exposure
Anti Rala Antibody, clone AB01/3E3 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of RALA from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RALA (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti Rala

Product Type
PrecisionAb Monoclonal
Clone
AB01/3E3
Isotype
IgG2a
Format
Purified
Specificity
Rala

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Product Code Applications Pack Size List Price Your Price Qty
VMA00772
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human RALA antibody recognizes the Ras-related protein Ral-A, also known as RAS-like protein A, Ras family small GTP binding protein RALA, ras related GTP binding protein A and ras-related protein Ral-A.

The product of RALA belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. RALA encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins (provided by RefSeq, Jul 2008).

Mouse anti Human RALA antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti RALA detects a band of approximately 24 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-206 of human RALA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P11233
Entrez Gene
RALA
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0000910 cytokinesis
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0007049 cell cycle
GO:0007265 Ras protein signal transduction
GO:0009986 cell surface
GO:0017157 regulation of exocytosis
GO:0030496 midbody
GO:0031532 actin cytoskeleton reorganization
GO:0031755 Edg-2 lysophosphatidic acid receptor binding
GO:0032154 cleavage furrow
GO:0044419 interspecies interaction between organisms
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051491 positive regulation of filopodium assembly
GO:0051665 membrane raft localization

VMA00772

149111

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