PRKAR2A antibody | OTI4A3

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Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PRKAR2A antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to PRKAR2A (molecular weight 51 kDa)

Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of PRKAR2A knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti PRKAR2A antibody (**VMA00718**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 375 second and (B) 30 second exposure.

Anti PRKAR2A Antibody, clone OTI4A3 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of PRKAR2A from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Mouse anti PRKAR2A

Product Type: PrecisionAb Monoclonal
Clone: OTI4A3
Isotype: IgG1
Format: Purified
Specificity: PRKAR2A

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00718 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human PRKAR2A antibody recognizes PRKAR2A, also known as PKR2, cAMP-dependent protein kinase regulatory subunit RII alpha and protein kinase A RII-alpha subunit.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by PRKAR2A is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) (provided by RefSeq, Jul 2008).

Mouse anti Human PRKAR2A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti PRKAR2A detects a band of approximately 51 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: HEK293-derived full length recombinant protein of human PRKAR2A
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P13861
Entrez Gene: PRKAR2A
GO Terms: GO:0001932 regulation of protein phosphorylation; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0035556 intracellular signal transduction; GO:0005624 membrane fraction; GO:0006112 energy reserve metabolic process; GO:0006833 water transport; GO:0007202 activation of phospholipase C activity; GO:0050796 regulation of insulin secretion; View More GO Terms; GO:0008603 cAMP-dependent protein kinase regulator activity; GO:0030552 cAMP binding; GO:0034199 activation of protein kinase A activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0055085 transmembrane transport; GO:0071377 cellular response to glucagon stimulus