PPP2CA antibody

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Mouse anti PPP2CA

PPP2CA antibody recognizes the Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C. The antibody has been extensively validated for western blotting.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00588 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human PPP2CA antibody recognizes the serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform or replication protein C.

PPP2CA gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA gene encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).

Mouse anti Human PPP2CA antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti PPP2CA detects a band of approximately 36 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites

Buffer Solution

HEPES buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol

Immunogen

Synthetic Peptide of human PPP2CA

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P67775 Related reagents
Entrez Gene: PPP2CA Related reagents
GO Terms: GO:0000159 protein phosphatase type 2A complex; GO:0000188 inactivation of MAPK activity; GO:0000775 chromosome, centromeric region; GO:0000922 spindle pole; GO:0005739 mitochondrion; GO:0005829 cytosol; GO:0004721 phosphoprotein phosphatase activity; GO:0046872 metal ion binding; GO:0005625 soluble fraction; GO:0005634 nucleus; GO:0006275 regulation of DNA replication; GO:0006470 protein dephosphorylation; GO:0006672 ceramide metabolic process; GO:0006917 induction of apoptosis; GO:0007126 meiosis; GO:0008380 RNA splicing; GO:0010033 response to organic substance; GO:0010719 negative regulation of epithelial to mesenchymal transition; GO:0019932 second-messenger-mediated signaling; GO:0030111 regulation of Wnt receptor signaling pathway; GO:0030155 regulation of cell adhesion; GO:0030308 negative regulation of cell growth; GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein; GO:0045449 regulation of transcription; GO:0071902 positive regulation of protein serine/threonine kinase activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PPP2CA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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