PPP2CA antibody
Mouse anti PPP2CA
PPP2CA antibody recognizes the Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C. The antibody has been extensively validated for western blotting.
PPP2CA Antibody with Control Lysate
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00588 | WB | 100 µl | ||||
| VMA00588KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human PPP2CA antibody recognizes the serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2A-alpha, protein phosphatase 2A catalytic subunit, alpha isoform or replication protein C.
PPP2CA gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA gene encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).
Mouse anti Human PPP2CA antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PPP2CA gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA gene encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).
Mouse anti Human PPP2CA antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PPP2CA detects a band of approximately 36 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
- Buffer Solution
- HEPES buffered saline
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- Synthetic Peptide of human PPP2CA
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P67775 Related reagents
- Entrez Gene
- PPP2CA Related reagents
- GO Terms
- GO:0000159 protein phosphatase type 2A complex
- GO:0000188 inactivation of MAPK activity
- GO:0000775 chromosome, centromeric region
- GO:0000922 spindle pole
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0004721 phosphoprotein phosphatase activity
- GO:0046872 metal ion binding
- GO:0005625 soluble fraction
- GO:0005634 nucleus
- GO:0006275 regulation of DNA replication
- GO:0006470 protein dephosphorylation
- GO:0006672 ceramide metabolic process
- GO:0006917 induction of apoptosis
- GO:0007126 meiosis
- GO:0008380 RNA splicing
- GO:0010033 response to organic substance
- GO:0010719 negative regulation of epithelial to mesenchymal transition
- GO:0019932 second-messenger-mediated signaling
- GO:0030111 regulation of Wnt receptor signaling pathway
- GO:0030155 regulation of cell adhesion
- GO:0030308 negative regulation of cell growth
- GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein
- GO:0045449 regulation of transcription
- GO:0071902 positive regulation of protein serine/threonine kinase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PPP2CA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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