PP1 Alpha antibody | OTI6E5

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Mouse anti PP1 Alpha

PP1 alpha antibody recognizes PP1 alpha, also known as serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit.

PP1 Alpha Antibody with Control Lysate

PP1 alpha antibody recognizes PP1 alpha, also known as serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI6E5
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00742 WB 100 µl
VMA00742KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PP1 alpha antibody recognizes PP1 alpha, also known as protein phosphatase 1-alpha isoform and serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit.

The protein encoded by PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for PPP1CA (provided by RefSeq, Jul 2008).

Mouse anti Human PP1 alpha antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti PP1 alpha detects a band of approximately 38 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
E. coli-derived full length recombinant protein of human PP1 alpha
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P62136 Related reagents
Entrez Gene
PPP1CA Related reagents
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0046872 metal ion binding
GO:0005730 nucleolus
GO:0005977 glycogen metabolic process
GO:0006470 protein dephosphorylation
GO:0007049 cell cycle
GO:0008599 protein phosphatase type 1 regulator activity
GO:0019433 triglyceride catabolic process
GO:0051301 cell division
GO:0070688 MLL5-L complex
GO:0072357 PTW/PP1 phosphatase complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PP1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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