POLY-ADP-RIBOSE antibody | AbD64138

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anti POLY-ADP-RIBOSE

Product Type: Monoclonal Antibody
Clone: AbD64138
Isotype: Fab antibody
Specificity: POLY-ADP-RIBOSE

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TZA0117 Datasheet SDS SDS	E IF WB	0.1 mg		Log in
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anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 1

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 2

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 3

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 4

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 5

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 6

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 7

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 8

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 9

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 10

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 11

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 12

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 13

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 thumbnail image 14

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 1 — **Published Originator image:**
Western blot analysis of recombinant poly-ADPr PARP1 probed with anti poly-ADP-ribose antibody, clone AbD64138 (**TZA0117**). Red represents oversaturated signal.
**Image caption:**
Modular Antibodies for versatile, specific and sensitive detection of Poly-ADPr. A: *In Vitro* modified recombinant PARP1, treated w/o PARG to create Poly-/ and Mono-ADPr PARP1 immunoblotted with new anti-Poly-ADPr antibodies in comparison to other available antibodies and reagents. Red represents oversaturated signal.

From: Dauben, H. & Matić, I. (2025)
Versatile Modular Antibodies for Sensitive and Specific Detection of Poly-ADP-Ribose.
**bioRxiv 2025.02.07.637082**.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. Western blots extracted from original figure and adapted to anonymize competitor reagents.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 2 — **Published Originator image:**
Western blot analysis of lysate prepared from H₂O₂ and Olaparib treated U2OS cells and probed with anti poly-ADP-ribose antibody, clone AbD64138 (**TZA0117**).
**Image caption:**
New highly sensitive Poly-ADPr-specific Antibodies. A: Immunoblotting of U2OS cells treated or not with H₂O₂, w/o Olaparib, lysed in SDS buffer and proceeded with immunoblotting. Antibodies and Reagents used in indicated concentration recommended by manufacturer or publication. HRP-format was used for new anti-poly-ADPr antibodies. Red represents oversaturated signal

From: Dauben, H. & Matić, I. (2025)
Versatile Modular Antibodies for Sensitive and Specific Detection of Poly-ADP-Ribose.
**bioRxiv 2025.02.07.637082**.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. Western blots extracted from original figure and adapted to show label and anonymize competitor reagents.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 3 — **Published Originator image:**
Western blot analysis of lysate prepared from H₂O₂ and Olaparib treated U2OS cells and probed with anti poly-ADP-ribose antibody, clone AbD64138 (**TZA0117**). Red represents oversaturated signal.
**Image caption:**
New highly sensitive Poly-ADPr-specific Antibodies. A: Immunoblotting of U2OS cells treated or not with H₂O₂, w/o Olaparib, lysed in SDS buffer and proceeded with immunoblotting. Antibodies and Reagents used in indicated concentration recommended by manufacturer or publication. HRP-format was used for new anti-poly-ADPr antibodies. Red represents oversaturated signal.

From: Dauben, H. & Matić, I. (2025)
Versatile Modular Antibodies for Sensitive and Specific Detection of Poly-ADP-Ribose.
**bioRxiv 2025.02.07.637082**.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. Western blots extracted from original figure and adapted to show label and anonymize competitor reagents.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 4 — **Published Originator image:**
U2OS cells damaged with H₂O₂ treatment and stained with anti poly-ADP-ribose antibody, clone AbD64138 (**TZA0117**), converted to synthetic IgG mouse format using mIgG2a-FcSpyCatcher3 (**TZC012**).
**Image caption:**
Versatile SpyTag System Enables Flexible Co-Detection of Mono- and Poly-ADPr in Cells. B: Immunofluorescence co-staining of mono- and poly-ADPr in the same cell using synthetic mouse/rabbit IgG antibody formats.

From: Dauben, H. & Matić, I. (2025)
Versatile Modular Antibodies for Sensitive and Specific Detection of Poly-ADP-Ribose.
**bioRxiv 2025.02.07.637082**.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. Immunofluorescence images extracted from original figure.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 5 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
G. Immunoblot analysis of SDS cell extracts from 2 mM H₂O₂-treated wild-type (WT) U2OS cells with and without 1 μM PARG inhibition treatment with the indicated antibodies. Detection was done in parallel, resulting in comparable signal intensities. Red colour represents a saturated signal. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 6 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
A. Immunoblot analysis of SDS cell extracts from 30 min 150 μM H2O2-treated followed by 1 h drug-free medium recovered wild-type (WT) and XRCC1KO hTERT RPE1 cells with the indicated antibodies. N = 4.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 7 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
C. Immunoblot analysis of SDS cell extracts from 150 μM H₂O₂-treated wild-type (WT) and XRCC1KO hTERT RPE1 cells with and without PARP1 inhibition (1 μM Olaparib or 10 nM AZD9574) for the indicated time points with the indicated antibodies. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 8 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
C. Immunoblot analysis of SDS cell extracts from 150 μM H₂O₂-treated wild-type (WT) and XRCC1KO hTERT RPE1 cells with and without PARP1 inhibition (1 μM Olaparib or 10 nM AZD9574) for the indicated time points with the indicated antibodies. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 9 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD43647 (**TZA020**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
D. Immunoblot analysis of isolated Histone H1 using three step perchloric acid (PCA) lysis of WT and XRCC1KO hTERT RPE1 cells after 30 min 150 μM H₂O₂-treated followed or not by 1 h drug free medium recovery. N = 3. E Immunoblot analysis of WT and XRCC1KO hTERT RPE1 cells after 30 min 150 μM H₂O₂-treated with H1.4-GFP overexpression. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 10 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) used to detect ADP ribose by immunofluorescence.
**Image caption:**
B Immunofluorescent co-staining of mono- and poly-ADPr in hTERT RPE1 WT or XRCC1KO cells followed by 150 μM H₂O₂ or 1.8 mM MMS treatment for the indicated time points. Left, quantitative analysis with error bars representing SEM. Signals are normalised to WT untreated conditions. Error bars represent SEM. Combined Analysis of 3 Biological Replicates (left). Representative Images from 1 Biological Replicate (right). Scale bar, 10 μM.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 11 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
C. Immunoblot analysis of SDS cell extracts from continuously 150 μM H₂O₂, 1.8 mM MMS or 15 μM CPT-treated wild-type (WT) and XRCC1KO hTERT RPE1 cells for the indicated time points with the indicated antibodies. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 12 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
A. Illustration showing the experimental set-up for full PARG inhibition (left top). Immunoblot analysis of SDS cell extracts from 150 μM H₂O₂-treated wild-type (WT) and XRCC1KO hTERT RPE1 cells with and without 1 μM PARG inhibition for the indicated time points with the indicated antibodies (right). N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 13 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
B. Illustration showing experimental set-up for pulse PARG inhibition (left top). Immunoblot analysis of SDS cell extracts from wild-type (WT) and XRCC1KO hTERT RPE1 cells with late PARG inhibition (10 μM) for the indicated time points before harvesting at 45 min after 150 μM H₂O₂-treatment with the indicated antibodies (right). N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 gallery image 14 — **Published customer image:**
Human anti poly ADP ribose antibody, clone AbD64138 (**TZA0117**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by Dot blotting.
**Image caption:**
(C) Quantification of Dot-blot analysis of *in vitro* autopoly-ADPr of PARP1 in 1:2 dilution series using Poly-/ Pan-ADPr antibodies. Combined Analysis of 3 Biological Replicates. Here mean gray values are presented without normalization, after background subtraction. Error bars represent SEM.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Filter by Application:

WB IF IB

Anti poly-ADP-ribose antibody, clone AbD64138, recognizes poly-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular organisms as well as some lower unicellular eukaryotes, but is absent in prokaryotes and yeast (Bürkle 2005). ADP ribosylation has been shown to play critical roles in many physiological and pathological processes, including bacterial pathogenesis and signaling and metabolism to control chromatin-related processes including transcription and DNA repair (Bonfiglio et al. 2020, Bütepage et al. 2015).

Members of the ADP-ribosyltransferase (ART) superfamily of proteins, including the poly(ADP-ribose) polymerases (PARPs) subfamily, catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) onto substrate protein via N-, O-, or S-glycosidic linkages. These transferases are able to transfer a single ADP-ribose residue to their substrate proteins, in a process known as mono-ADP-ribosylation. They are also able to attach additional ADP-ribose residues to create either linear or branched chains of ADP-ribose, known as poly-ADP-ribosylation (Poly-ADPr) (Bütepage et al. 2015).

This anti poly-ADP-ribose antibody, clone AbD64138, specifically and sensitively recognizes poly-ADPr but not mono-ADPr. Clone AbD64138 is tagged with a SpyTag at the C-terminus of the Fab heavy chain, enabling the user to couple the purified format to a SpyCatcher reagent for conversion to alternative formats in less than an hour.

Target Species: Human
Product Form: A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation: Recombinant Fab antibody expressed in E. coli and purified by affinity chromatography
Source: E. coli.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.0095% MIT
Carrier Free: Yes
Immunogen: ARTKQTARKS(polyADPr)TGGKAC
Approx. Protein Concentrations: Total protein concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: Bio-Rad has obtained the right for the manufacture and commercialization of the antibodies from Max-Planck-Innovation GmbH, the technology transfer office of the Max Planck Institute for Biology of Ageing. Further information on the Serine ADP-ribosylation technology used to generate and characterize the antibodies can be found here: Dauber et al. 2023 and Bonfiglio et al. 2020.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunofluorescence
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Source Reference

Dauben, H. & Matić, I. (2025) Versatile Modular Antibodies for Sensitive and Specific Detection of Poly-ADP-Ribose
bioRχiv 10 Feb [Preprint].

References for POLY-ADP-RIBOSE antibody

Dauben, H. et al. (2026) Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
Nat Commun. 17 (1): 3216.