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PDGF-BB antibody | AB13-3G6

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Anti Human PDGF-BB Antibody, clone AB13-3G6 thumbnail image 1
Anti Human PDGF-BB Antibody, clone AB13-3G6 gallery image 1
Sandwich ELISA analysis of human PDGF-BB using Mouse anti Human PDGF-BB (MCA6264GA) as a capture reagent and biotinylated Mouse anti Human PDGF-BB (MCA6265B) as a detection reagent with purified recombinant PDGF-BB as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human PDGF-BB:Biotin

Product Type
Monoclonal Antibody
Clone
AB13-3G6
Isotype
IgG2b
Specificity
PDGF-BB

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Product Code Applications Pack Size List Price Your Price Qty
MCA6265B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human PDGF-BB antibody, clone AB13-3G6, recognizes the homodimer of platelet-derived growth factor subunit B (PDGF-B) also known as PDGF-2, PDGF beta chain and PDGF beta polypeptide. PDGF-BB is a member of the PDGF growth factor family which is made up of 4 isoforms (A, B, C and D). PDGF-BB is a homodimer of the B isoform which is able to bind all three of the PDGF receptors to trigger various signaling pathways including Ras-MAPK, PI3K, PLCγ, and JAK (Evrova & Buschmann 2017). Activation of these pathways lead to transcription of PDGF target genes playing roles in embryogenesis, and in adults under physiological conditions, roles in tissue repair, wound healing and maturation of blood vessels and white adipocytes (Saplacan et al. 2017).

The biotinylated Mouse anti Human PDGF-BB antibody, clone AB13-3G6 (MCA6265B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human PDGF-BB antibody, clone AB06-1E10 (MCA6264GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length PDGF-BB (Ser82-Thr190)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6264GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/lm
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P01127
Entrez Gene
PDGFB
GO Terms
GO:0000139 Golgi membrane
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002548 monocyte chemotaxis
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005161 platelet-derived growth factor receptor binding
GO:0005518 collagen binding
GO:0005788 endoplasmic reticulum lumen
GO:0008083 growth factor activity
GO:0009986 cell surface
GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
GO:0010544 negative regulation of platelet activation
GO:0014911 positive regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0042803 protein homodimerization activity
GO:0043406 positive regulation of MAP kinase activity
GO:0043498 cell surface binding
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0045740 positive regulation of DNA replication
GO:0046982 protein heterodimerization activity
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
GO:0050921 positive regulation of chemotaxis
GO:0051781 positive regulation of cell division

MCA6265B

100005419

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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