NGAL antibody | D02-7D4
Mouse anti Human NGAL:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- D02-7D4
- Isotype
- IgG1
- Specificity
- NGAL
| Mouse anti Human NGAL antibody, clone D02-7D4, recognizes Neutrophil Gelatinase-Associated Lipocalin (NGAL) also known as lipocalin-2, 24 kDa alpha-2-microglobin-related subunit of MMP-9 and siderocalin. NGAL is a 198 amino acid 25 kDa secreted protein which possesses a lipid binding domain which enables it to bind small hydrophobic ligand molecules such as bacterial siderophores (Bauvois & Susin 2018, Abella et al. 2015). As well as the 25 kDa form, NGAL can exist as a 46 kDa homodimer and a 130 kDa heterodimer with MMP-9. The interaction with MMP-9 occurs in the extracellular space upon secretion of both proteins. It is through this interaction that MMP-9 proteolytic activity can be inhibited (Chakraborty et al. 2012). NGAL is expressed in various cell types including adipocytes, hepatocytes, pneumocytes, splenocytes, mesangial and microglial cells. NGAL has also been observed circulating at low levels in urine and blood of healthy subjects (40-109 ng/ml) . However it is overexpressed during various inflammatory, metabolic, neurologic and cancer pathologies. The higher levels of NGAL expression in these diseases have been correlated with disease severity and poor prognosis (Bauvois & Susin 2018, Chakraborty et al. 2012). The biotinylated Mouse anti Human NGAL antibody, clone D02-7D4 (MCA6199B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human NGAL antibody, clone D11-6D10 (MCA6198GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli derived recombinant human NGAL (Gln21-Gly198)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6198GA
ELISA Antigen-Positive Control:
PHP302
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human NGAL | MCA6198GA | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Human NGAL | ||||||
| Recombinant Human NGAL | PHP302 | E | 10 µg | Log in | |||
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| Description | Recombinant Human NGAL | ||||||
MCA6199B
100005426
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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