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NFkB p50 antibody

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Anti NFkB p50 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
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Western blot analysis of whole cell lysates probed with NFkB p50 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to NFkB p50 (molecular weight 50 & 120 kDa).

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WB

Mouse anti NFkB p50

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
NFkB p50

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VMA00673
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WB 100 µl loader
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Mouse anti Human NFkB p50 antibody recognizes nuclear factor NF-kappa-B p50 subunit, also known as DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit or nuclear factor kappa-B DNA binding subunit.

NFKB1 encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for NFKB1 (provided by RefSeq, Sep 2009).

Mouse anti Human NFkB p50 antibody detects bands of 50 and 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti NFkB p50 antibody detects bands of approximately 55 and 120 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant human NFkB p50 (aa 1-200)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19838
Entrez Gene
NFKB1
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006366 transcription from RNA polymerase II promoter
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010843 promoter binding
GO:0010884 positive regulation of lipid storage
GO:0010956 negative regulation of calcidiol 1-monooxygenase activity
GO:0010957 negative regulation of vitamin D biosynthetic process
GO:0016563 transcription activator activity
GO:0031293 membrane protein intracellular domain proteolysis
GO:0032269 negative regulation of cellular protein metabolic process
GO:0032375 negative regulation of cholesterol transport
GO:0033256 I-kappaB/NF-kappaB complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway

VMA00673

170504

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