NFkB p50 antibody

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Mouse anti NFkB p50

NFkB p50 antibody recognizes nuclear factor NF-kappa-B p50 subunit, also known as DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit or nuclear factor kappa-B DNA binding subunit.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00673 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human NFkB p50 antibody recognizes nuclear factor NF-kappa-B p50 subunit, also known as DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit or nuclear factor kappa-B DNA binding subunit.

NFKB1 encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for NFKB1 (provided by RefSeq, Sep 2009).

Mouse anti Human NFkB p50 antibody detects bands of 50 and 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Mouse anti NFkB p50 antibody detects bands of approximately 55 and 120 kDa in Raji cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Recombinant human NFkB p50 (aa 1-200)
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: P19838 Related reagents
Entrez Gene: NFKB1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006915 apoptosis; GO:0005739 mitochondrion; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0006366 transcription from RNA polymerase II promoter; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0008063 Toll signaling pathway; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010744 positive regulation of macrophage derived foam cell differentiation; GO:0010843 promoter binding; GO:0010884 positive regulation of lipid storage; GO:0010956 negative regulation of calcidiol 1-monooxygenase activity; GO:0010957 negative regulation of vitamin D biosynthetic process; GO:0016563 transcription activator activity; GO:0031293 membrane protein intracellular domain proteolysis; GO:0032269 negative regulation of cellular protein metabolic process; GO:0032375 negative regulation of cholesterol transport; GO:0033256 I-kappaB/NF-kappaB complex; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of NFkB p50 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

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