Myosin Heavy Chain 10 antibody | AB01-2/4F2

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Mouse anti Myosin Heavy Chain 10

Product Type
PrecisionAb Monoclonal
Clone
AB01-2/4F2
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00783
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human myosin heavy chain 10 antibody recognizes myosin heavy chain 10, also known as cellular myosin heavy chain type B, myosin heavy polypeptide 10 non-muscle and myosin-10.

MYH10 encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in MYH10 have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for MYH10 (provided by RefSeq, Dec 2011).

Mouse anti Human myosin heavy chain 10 antibody detects a band of 248 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti myosin heavy chain 10 detects a band of approximately 248 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1580-1976 of human myosin heavy chain 10

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P35580
Entrez Gene
MYH10
GO Terms
GO:0000146 microfilament motor activity
GO:0000281 cytokinesis after mitosis
GO:0001725 stress fiber
GO:0051015 actin filament binding
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0005938 cell cortex
GO:0007411 axon guidance
GO:0008360 regulation of cell shape
GO:0030048 actin filament-based movement
GO:0016459 myosin complex
GO:0030496 midbody
GO:0030898 actin-dependent ATPase activity
GO:0032154 cleavage furrow
GO:0043531 ADP binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Myosin Heavy Chain 10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml