Mucin 2 antibody | 996/1
Mouse anti Human Mucin 2:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 996/1
- Isotype
- IgG1
- Specificity
- Mucin 2
Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes human Mucin 2 (MUC-2), and shows no cross-reactivity with MUC-1, MUC-3 or MUC-4. In formalin fixed, paraffin embedded tissue sections Mouse anti Human Mucin 2 antibody, clone 996/1 reveals high levels of expression in colon, liver and prostate tissues (Durrant et al. 1994). Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes malignant colonic mucosa as well as normal mucosa. Epitope mapping indicates that Mouse anti Human Mucin 2 antibody, clone 996/1 recognizes a sequence PTGTQ within the mucin 2 tandem repeat region (Uray et al. 1999). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- MUC-2 tandem repeat peptide.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of a mouse NS0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | Neat | 1/10 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
Source Reference
-
Durrant, L.G. et al. (1994) Production of monoclonal antibodies recognising the peptide core of MUC2 intestinal mucin.
Eur J Cancer. 30A (3): 355-63.
References for Mucin 2 antibody
-
Price, M.R. et al. (1993) Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody.
Int J Cancer. 55 (5): 753-9. -
Filipe, M.I. et al. (1996) Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma.
Eur J Cancer Prev. 5 (4): 287-95. -
Paulsen, F.P. et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct.
Invest Ophthalmol Vis Sci. 44 (5): 1807-13. -
Price, M.R. et al. (1999) Separation of distinct MUC2 mucin glycoforms using two anti-peptide monoclonal antibodies.
Int J Oncol. 15 (4): 803-9. -
Tugyi, R. et al. (2005) Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.
Proc Natl Acad Sci U S A. 102 (2): 413-8. -
Uray, K. et al. (2000) Effect of D-amino acid substitution in a mucin 2 epitope on mucin-specific monoclonal antibody recognition.
Arch Biochem Biophys. 378 (1): 25-32. -
Uray, K. et al. (1999) Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2).
Arch Biochem Biophys. 361 (1): 65-74.
- Synonyms
- MUC-2
- UniProt
- Q02817
- Entrez Gene
- MUC2
- GO Terms
- GO:0005515 protein binding
- GO:0070702 inner mucus layer
- GO:0070703 outer mucus layer
MCA1743A647
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