MRE11 antibody | AB02/1D11
Mouse anti Human MRE11 antibody recognizes the double-strand break repair protein MRE11A, also known as AT-like disease DNA recombination and repair protein, MRE11 homolog 1, MRE11 homolog A, endo/exonuclease Mre11 or meiotic recombination 11 homolog A.
MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).
Mouse anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).
Mouse anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti MRE11 detects a band of approximately 81 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human MRE11A (amino acids 182 - 582)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P49959 Related reagents
- Entrez Gene
- MRE11A Related reagents
- GO Terms
- GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity
- GO:0000019 regulation of mitotic recombination
- GO:0000724 double-strand break repair via homologous recombination
- GO:0003690 double-stranded DNA binding
- GO:0005654 nucleoplasm
- GO:0006303 double-strand break repair via nonhomologous end joining
- GO:0007004 telomere maintenance via telomerase
- GO:0007062 sister chromatid cohesion
- GO:0007131 reciprocal meiotic recombination
- GO:0008022 protein C-terminus binding
- GO:0008408 3'-5' exonuclease activity
- GO:0030145 manganese ion binding
- GO:0030870 Mre11 complex
- GO:0031954 positive regulation of protein autophosphorylation
- GO:0032508 DNA duplex unwinding
- GO:0032876 negative regulation of DNA endoreduplication
- GO:0033674 positive regulation of kinase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of MRE11 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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