MRE11 antibody | AB02/1D11

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Mouse anti MRE11

MRE11 Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Clone
AB02/1D11
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00536 WB 100 µl
VMA00536KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human MRE11 antibody recognizes the double-strand break repair protein MRE11A, also known as AT-like disease DNA recombination and repair protein, MRE11 homolog 1, MRE11 homolog A, endo/exonuclease Mre11 or meiotic recombination 11 homolog A.

MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Mouse anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MRE11 detects a band of approximately 81 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human MRE11A (amino acids 182 - 582)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P49959 Related reagents
Entrez Gene
MRE11A Related reagents
GO Terms
GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity
GO:0000019 regulation of mitotic recombination
GO:0000724 double-strand break repair via homologous recombination
GO:0003690 double-stranded DNA binding
GO:0005654 nucleoplasm
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0007004 telomere maintenance via telomerase
GO:0007062 sister chromatid cohesion
GO:0007131 reciprocal meiotic recombination
GO:0008022 protein C-terminus binding
GO:0008408 3'-5' exonuclease activity
GO:0030145 manganese ion binding
GO:0030870 Mre11 complex
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0032876 negative regulation of DNA endoreduplication
GO:0033674 positive regulation of kinase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MRE11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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