MRE11 antibody | AB02/1D11

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Anti MRE11 Antibody, clone AB02/1D11 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MRE11 Antibody, clone AB02/1D11 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MRE11 Antibody, clone AB02/1D11 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MRE11 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 118 second exposure. Arrow points to MRE11 (molecular weight 81 kDa)

Anti MRE11 Antibody, clone AB02/1D11 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of MRE11 from HEK293 whole cell lysate. The Input lane is 10% input; the MRE11-IP lane is the immunoprecipitation with the MRE11 antibody (2.5 µg, **VMA00536**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using MRE11 antibody (1/1,000, **VMA00536**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to MRE11.

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WB IP

Mouse anti MRE11

Product Type: PrecisionAb Monoclonal
Clone: AB02/1D11
Isotype: IgG1
Format: Purified
Specificity: MRE11

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00536 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human MRE11 antibody recognizes the double-strand break repair protein MRE11A, also known as AT-like disease DNA recombination and repair protein, MRE11 homolog 1, MRE11 homolog A, endo/exonuclease Mre11 or meiotic recombination 11 homolog A.

MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Mouse anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MRE11 detects a band of approximately 81 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human MRE11A (amino acids 182 - 582)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P49959
Entrez Gene: MRE11A
GO Terms: GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity; GO:0000019 regulation of mitotic recombination; GO:0000724 double-strand break repair via homologous recombination; GO:0003690 double-stranded DNA binding; GO:0005654 nucleoplasm; GO:0006303 double-strand break repair via nonhomologous end joining; GO:0007004 telomere maintenance via telomerase; GO:0007062 sister chromatid cohesion; GO:0007131 reciprocal meiotic recombination; View More GO Terms; GO:0008022 protein C-terminus binding; GO:0008408 3'-5' exonuclease activity; GO:0030145 manganese ion binding; GO:0030870 Mre11 complex; GO:0031954 positive regulation of protein autophosphorylation; GO:0032508 DNA duplex unwinding; GO:0032876 negative regulation of DNA endoreduplication; GO:0033674 positive regulation of kinase activity