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MMP-9 antibody | B10-7B9

Mouse anti Human MMP-9:Biotin

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Mouse anti Human MMP-9 antibody, clone B10-7B9, recognizes matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form (Vandooren et al. 2013). Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling.

Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.

The biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) as the capture antibody.

Target Species
Product Form
Purified IgG conjugated to Biotin - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
E. coli derived recombinant fragment of human MMP-9 (Gly373-Pro456)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6196GA
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human MMP-9 MCA6196GA E 0.1 mg loader
List Price Your Price
Description Mouse anti Human MMP-9

Matrix metalloproteinase-9
Entrez Gene
GO Terms
GO:0004222 metalloendopeptidase activity
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030225 macrophage differentiation
GO:0030574 collagen catabolic process
GO:0051549 positive regulation of keratinocyte migration



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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