Megalin antibody | AbD11723

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Human anti Human Megalin

Product Type
Monoclonal Antibody
Clone
AbD11723
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA146 P 0.1 mg
Human anti Human megalin antibody, clone AbD11723 recognizes human megalin, also known as LRP-2 and Glycoprotein 330, a transmembrane protein with an approximate molecular weight of 521 kDa belonging to the LDLR family.

Megalin is a multi-ligand binding receptor involved in the mediation of various cell processes including the binding of plasminogen, lactoferin and various extracellular matrix components. In association with RAP, megalin forms the Heymann nephritis antigenic complex. Magalin is located in absorptive epithelia including the renal proximal tubules.

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with V5 and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human megalin (aa sequence 1024-1224) – N1 fusion protein.
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P98164 Related reagents
Entrez Gene
LRP2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005764 lysosome
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005905 coated pit
GO:0006486 protein glycosylation
GO:0006898 receptor-mediated endocytosis
GO:0017124 SH3 domain binding
GO:0042359 vitamin D metabolic process
GO:0042446 hormone biosynthetic process
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Megalin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1:50 1:100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti V5-Tag MCA1360 1 mg C E F IF IP R WB
Mouse anti V5-Tag:Alk. Phos. MCA1360A 0.1 mg E WB
Mouse anti Strep-Tag Immo MCA2488 0.1 mg E

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P