Megalin antibody | AbD11723

Human anti Human Megalin

Product Type
Monoclonal Antibody
Clone
AbD11723
Isotype
HuCAL Fab bivalent
Specificity
Megalin

Product Code Applications Pack Size List Price Your Price Qty
HCA146
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P 0.1 mg loader
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Human anti Human megalin antibody, clone AbD11723 recognizes human megalin, also known as LRP-2 and Glycoprotein 330, a transmembrane protein with an approximate molecular weight of 521 kDa belonging to the LDLR family.

Megalin is a multi-ligand binding receptor involved in the mediation of various cell processes including the binding of plasminogen, lactoferin and various extracellular matrix components. In association with RAP, megalin forms the Heymann nephritis antigenic complex. Magalin is located in absorptive epithelia including the renal proximal tubules.

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
StrepTactin affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Human megalin (aa sequence 1024-1224) – N1 fusion protein.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1:250 1:500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Histology Positive Control Tissue
Human kidney.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti V5-Tag MCA1360 C E F IF IP R WB 1 mg loader
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Description Mouse anti V5-Tag
Mouse anti V5-Tag:Alk. Phos. MCA1360A E WB 0.1 mg loader
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Description Mouse anti V5-Tag:Alk. Phos.
Mouse anti Strep-Tag Immo MCA2488 E 0.1 mg loader
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Description Mouse anti Strep-Tag Immo

RRID
AB_10718708
UniProt
P98164
Entrez Gene
LRP2
GO Terms
GO:0005509 calcium ion binding
GO:0005764 lysosome
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005905 coated pit
GO:0006486 protein glycosylation
GO:0006898 receptor-mediated endocytosis
GO:0017124 SH3 domain binding
GO:0042359 vitamin D metabolic process
GO:0042446 hormone biosynthetic process
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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