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MCM7 antibody | AB04/3F1

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siRNA Validated
IP-MS Validated
Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MCM7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MCM7 (molecular weight 81 kDa)
Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against MCM7 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti MCM7 antibody (VMA00544) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 86 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of MCM7 from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against MCM7 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.
Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of MCM7 from HeLa whole cell lysate. The Input lane is 10% input; the MCM7-IP lane is the immunoprecipitation with the MCM7 antibody (2.5 µg, VMA00544); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using MCM7 antibody (1/1,000, VMA00544) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MCM7.

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WB IP

Mouse anti MCM7

Product Type
PrecisionAb Monoclonal
Clone
AB04/3F1
Isotype
IgG1
Format
Purified
Specificity
MCM7

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VMA00544
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IP WB 100 µl loader
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Mouse anti Human MCM7 antibody recognizes the DNA replication licensing factor MCM7, also known as CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7 or protein phosphatase 1, regulatory subunit 104.

The protein encoded by MCM7 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human MCM7 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MCM7 detects a band of approximately 81 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human MCM7 (amino acids 1 - 390)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P33993
Entrez Gene
MCM7
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000785 chromatin
GO:0005524 ATP binding
GO:0006270 DNA-dependent DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006350 transcription
GO:0006974 response to DNA damage stimulus
GO:0042325 regulation of phosphorylation
GO:0042555 MCM complex
GO:0045449 regulation of transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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