MCM7 antibody | AB04/3F1

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Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MCM7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MCM7 (molecular weight 81 kDa)

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against MCM7 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti MCM7 antibody (**VMA00544**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 86 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of MCM7 from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against MCM7 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb Monoclonal Antibody) gallery image 4 — Immunoprecipitation of MCM7 from HeLa whole cell lysate. The Input lane is 10% input; the MCM7-IP lane is the immunoprecipitation with the MCM7 antibody (2.5 µg, **VMA00544**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using MCM7 antibody (1/1,000, **VMA00544**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MCM7.

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WB IP

Mouse anti MCM7

Product Type: PrecisionAb Monoclonal
Clone: AB04/3F1
Isotype: IgG1
Format: Purified
Specificity: MCM7

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VMA00544 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human MCM7 antibody recognizes the DNA replication licensing factor MCM7, also known as CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7 or protein phosphatase 1, regulatory subunit 104.

The protein encoded by MCM7 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human MCM7 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MCM7 detects a band of approximately 81 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human MCM7 (amino acids 1 - 390)
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P33993
Entrez Gene: MCM7
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000785 chromatin; GO:0005524 ATP binding; GO:0006270 DNA-dependent DNA replication initiation; GO:0006271 DNA strand elongation involved in DNA replication; View More GO Terms; GO:0006350 transcription; GO:0006974 response to DNA damage stimulus; GO:0042325 regulation of phosphorylation; GO:0042555 MCM complex; GO:0045449 regulation of transcription