MCM7 antibody | AB04/3F1

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Anti MCM7 Antibody, clone AB04/3F1 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MCM7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MCM7 (molecular weight 81 kDa)

Mouse anti MCM7

MCM7 antibody recognizes the DNA replication licensing factor MCM7, also known as CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7, protein phosphatase 1, regulatory subunit 104. The antibody has been extensively validated for western blotting.

MCM7 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: AB04/3F1
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00544	WB	100 µl
VMA00544KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human MCM7 antibody recognizes the DNA replication licensing factor MCM7, also known as CDC47 homolog, homolog of S. cerevisiae Cdc47, minichromosome maintenance deficient 7 or protein phosphatase 1, regulatory subunit 104.

The protein encoded by MCM7 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human MCM7 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli-derived recombinant human MCM7 (amino acids 1 - 390)

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P33993 Related reagents
Entrez Gene: MCM7 Related reagents
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000785 chromatin; GO:0005524 ATP binding; GO:0006270 DNA-dependent DNA replication initiation; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006350 transcription; GO:0006974 response to DNA damage stimulus; GO:0042325 regulation of phosphorylation; GO:0042555 MCM complex; GO:0045449 regulation of transcription
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of MCM7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti MCM7 detects a band of approximately 81 kDa in HeLa cell lysates
Western Blotting: Anti MCM7 detects a band of approximately 81 kDa in HeLa cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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