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Laminin antibody | 4C7 (2D8/33)

Mouse anti Human Laminin Alpha 5

Product Type
Monoclonal Antibody
4C7 (2D8/33)

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Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 recognizes the laminin alpha 5 chain also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha or Laminin-15 subunit alpha. Laminin is a complex glycoprotein composed of three polypeptide chain complexes. Laminin alpha 5 is expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta, there is little or no expression in brain and liver.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites - liquid
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Purified human laminin.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the mouse X63-Ag8.563 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Source Reference

  1. Engvall, E. et al. (1986) Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
    J Cell Biol. 103 (6 Pt 1): 2457-65.

References for Laminin antibody

  1. Engvall, E. et al. (1990) Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Cell Regul. 1 (10): 731-40.
  2. Ido, H. et al. (2006) Probing the integrin-binding site within the globular domain of laminin-511 with the function-blocking monoclonal antibody 4C7.
    Matrix Biol. 25 (2): 112-7.
  3. Wondimu, Z. et al. (2013) A novel monoclonal antibody to human laminin α5 chain strongly inhibits integrin-mediated cell adhesion and migration on laminins 511 and 521.
    PLoS One. 8 (1): e53648.
  4. Vuoristo, S. et al. (2013) A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation.
    PLoS One. 8 (10): e76205.
  5. Pouliot, N. & Kusuma, N. (2013) Laminin-511: a multi-functional adhesion protein regulating cell migration, tumor invasion and metastasis.
    Cell Adh Migr. 7 (1): 142-9.
  6. Zamurs, L. et al. (2013) Chain-specific antibodies for laminin-511.
    Growth Factors. 31 (6): 209-19.
  7. Chang, C. et al. (2015) A laminin 511 matrix is regulated by TAZ and functions as the ligand for the α6Bβ1 integrin to sustain breast cancer stem cells.
    Genes Dev. 29 (1): 1-6.

Further Reading

  1. Breitkreutz, D. et al. (2013) Skin basement membrane: the foundation of epidermal integrity--BM functions and diverse roles of bridging molecules nidogen and perlecan.
    Biomed Res Int. 2013: 179784.


Immunohistology - Frozen

Entrez Gene
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0004872 receptor activity
GO:0005178 integrin binding
GO:0005198 structural molecule activity
GO:0005606 laminin-1 complex
GO:0005615 extracellular space
GO:0007229 integrin-mediated signaling pathway
GO:0008037 cell recognition
GO:0008283 cell proliferation
GO:0030155 regulation of cell adhesion
GO:0030334 regulation of cell migration
GO:0034446 substrate adhesion-dependent cell spreading
GO:0043259 laminin-10 complex
GO:0043260 laminin-11 complex
GO:0045446 endothelial cell differentiation
GO:0045995 regulation of embryonic development
GO:0048041 focal adhesion assembly


151167 153772

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