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Laminin antibody | 4C7 (2D8/33)

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Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) thumbnail image 1 Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) thumbnail image 2 Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) thumbnail image 3
Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) gallery image 1
Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the identification of alpha-5 laminin from JAR choriocarcinoma cells by immunopercipitation.
Image caption:
Expression and synthesis of laminins by JAR cells.
A) JAR choriocarcinoma cells transcribe lm α1, lm α3, lm α5, lm β1, lm β2, lm β, lm γ2, and lm γ1 chains as shown by RT-PCR. As a reference sample, we used optimized, in-house prepared positive control mix, which contains cDNA from undifferentiated hPSCs, spontaneously differentiated hPSCs as well as from isolated human pancreatic islets. B) JAR choriocarcinoma cells were metabolically labeled with 35S and the cell culture supernatant and matrix deposited by JAR cells were immunoprecipitated using antibodies, which specifically recognize the human lm α1 (400 kDa) and lm α5 (350-380 kDa) chains. The cells showed abundant lm-511 and -111 synthesis.

From: Vuoristo S, Toivonen S, Weltner J, Mikkola M, Ustinov J, Trokovic R, et al. (2013)
A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation.
PLoS ONE 8(10): e76205.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) gallery image 2
Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the identification of lamanin alpha-5 by western blotting.
Image caption:
Reactivity of novel mAbs to LMα5 chain as measured by ELISA, Western blotting and immunoprecipitation. A) Reactivity of the antibodies with rhLMs 411, 511 and 521 by ELISA. B) Reactivity of the antibodies with rhLM511 by Western blotting under reducing conditions. C) Ability of the antibodies to immunoprecipitate laminin-511 from A549 cells' conditioned medium and detection of the LMα5 chain by Western blotting with mAb 4B5. Bands of 300/350 kDa corresponding to LMα5 chain were detected with some of the antibodies.

From: Citation: Wondimu Z, Omrani S, Ishikawa T, Javed F, Oikawa Y, Virtanen I, et al. (2013)
A Novel Monoclonal Antibody to Human Laminin α5 Chain Strongly Inhibits Integrin-Mediated Cell Adhesion and Migration on Laminins 511 and 521.
PLoS ONE 8(1): e53648.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Laminin Alpha 5 Antibody, clone 4C7 (2D8/33) gallery image 3
Published investigator image:
Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 (5620-0436) used for the evaluation of lamanin alpha-5 expression in human kidney by immunohistofluorescence on acetone fixed cryosections.
Image caption:
Immunohistochemical reactivity of mAb 8G9 in comparison to mAb 4C7 against LMα5 chain.
(A) and (C), mAb 4C7; (B) and (D), mAb 8G9 against frozen sections of human kidney (A) and (B), and skin (C) and (D). The staining pattern of the two antibodies was indistinguishable. In kidney, the antibodies stained glomerular and tubular basement membranes, whereas in skin, reactivity with epidermal and vascular basement membranes was observed.

From: Citation: Wondimu Z, Omrani S, Ishikawa T, Javed F, Oikawa Y, Virtanen I, et al. (2013)
A Novel Monoclonal Antibody to Human Laminin α5 Chain Strongly Inhibits Integrin-Mediated Cell Adhesion and Migration on Laminins 511 and 521.
PLoS ONE 8(1): e53648.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human Laminin Alpha 5

Product Type
Monoclonal Antibody
Clone
4C7 (2D8/33)
Isotype
IgG2a
Specificity
Laminin
Region
ALPHA 5

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5620-0436
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Mouse anti Human Laminin Alpha 5 antibody, clone 4C7 recognizes the laminin alpha 5 chain also known as Laminin-10 subunit alpha, Laminin-11 subunit alpha or Laminin-15 subunit alpha. Laminin is a complex glycoprotein composed of three polypeptide chain complexes. Laminin alpha 5 is expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta, there is little or no expression in brain and liver.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites - liquid
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified human laminin.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Laminin antibody

  1. Engvall, E. et al. (1986) Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
    J Cell Biol. 103 (6 Pt 1): 2457-65.
  2. Engvall, E. et al. (1990) Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Cell Regul. 1 (10): 731-40.
  3. Ido, H. et al. (2006) Probing the integrin-binding site within the globular domain of laminin-511 with the function-blocking monoclonal antibody 4C7.
    Matrix Biol. 25 (2): 112-7.
  4. Chang, C. et al. (2015) A laminin 511 matrix is regulated by TAZ and functions as the ligand for the α6Bβ1 integrin to sustain breast cancer stem cells.
    Genes Dev. 29 (1): 1-6.
  5. Zamurs, L. et al. (2013) Chain-specific antibodies for laminin-511.
    Growth Factors. 31 (6): 209-19.
  6. Tiger, C.F. et al. (1997) Presence of laminin alpha5 chain and lack of laminin alpha1 chain during human muscle development and in muscular dystrophies.
    J Biol Chem. 272 (45): 28590-5.
  7. Pouliot, N. & Kusuma, N. (2013) Laminin-511: a multi-functional adhesion protein regulating cell migration, tumor invasion and metastasis.
    Cell Adh Migr. 7 (1): 142-9.
  8. Schaff, M. et al. (2013) Integrin α6β1 is the main receptor for vascular laminins and plays a role in platelet adhesion, activation, and arterial thrombosis.
    Circulation. 128 (5): 541-52.
    9. View The Latest Product References
  9. Vuoristo, S. et al. (2013) A novel feeder-free culture system for human pluripotent stem cell culture and induced pluripotent stem cell derivation.
    PLoS One. 8 (10): e76205.
  10. Wondimu, Z. et al. (2013) A novel monoclonal antibody to human laminin α5 chain strongly inhibits integrin-mediated cell adhesion and migration on laminins 511 and 521.
    PLoS One. 8 (1): e53648.
  11. Breitkreutz, D. et al. (2013) Skin basement membrane: the foundation of epidermal integrity--BM functions and diverse roles of bridging molecules nidogen and perlecan.
    Biomed Res Int. 2013: 179784.

Immunofluorescence

Immunohistology - Frozen

RRID
AB_617326
UniProt
O15230
Entrez Gene
LAMA5
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0004872 receptor activity
GO:0005178 integrin binding
GO:0005198 structural molecule activity
GO:0005606 laminin-1 complex
GO:0005615 extracellular space
GO:0007229 integrin-mediated signaling pathway
GO:0008037 cell recognition
GO:0008283 cell proliferation
GO:0030155 regulation of cell adhesion
GO:0030334 regulation of cell migration
GO:0034446 substrate adhesion-dependent cell spreading
GO:0043259 laminin-10 complex
GO:0043260 laminin-11 complex
GO:0045446 endothelial cell differentiation
GO:0045995 regulation of embryonic development
GO:0048041 focal adhesion assembly
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