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KIR antibody | NKVFS1

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Anti Human KIR Antibody, clone NKVFS1 thumbnail image 1 Anti Human KIR Antibody, clone NKVFS1 thumbnail image 2
Anti Human KIR Antibody, clone NKVFS1 gallery image 1
Figure A. FITC conjugated mouse anti human CD3 (MCA2184F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti human CD3 (MCA2184F) and RPE conjugated mouse anti human CD158a (MCA2243PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human KIR Antibody, clone NKVFS1 gallery image 2
Figure A. Alexa647® conjugated mouse anti human CD56 (MCA2693A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa647® conjugated mouse anti human CD56 (MCA2693A647) and RPE conjugated mouse anti human CD158a (MCA2243PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Mouse anti Human KIR:RPE

Product Type
Monoclonal Antibody
Clone
NKVFS1
Isotype
IgG1
Specificity
KIR

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Product Code Applications Pack Size List Price Your Price Qty
MCA2243PE
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F 100 Tests loader
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Mouse anti Human KIR antibody, clone NKVFS1 recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3. KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.

Mouse anti Human KIR antibody, clone NKVFS1 recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4).

Mouse anti Human KIR antibody, clone NKVFS1 is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms..

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for KIR antibody

  1. Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
    Blood. 99 (5): 1706-14.
  2. Spaggiari, G.M. et al. (2002) Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction.
    Blood. 100 (12): 4098-107.
  3. Patterson, S. et al. (2008) Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors.
    J Immunol. 181 (5): 3268-76.
  4. Moesta, A.K. et al. (2009) Chimpanzees use more varied receptors and ligands than humans for inhibitory killer cell Ig-like receptor recognition of the MHC-C1 and MHC-C2 epitopes.
    J Immunol. 182 (6): 3628-37.
  5. Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
    Immunol Invest. 38 (5): 434-46.
  6. Older Aguilar, A.M. et al. (2010) Coevolution of killer cell Ig-like receptors with HLA-C to become the major variable regulators of human NK cells.
    J Immunol. 185 (7): 4238-51.
  7. Hilton, H.G. et al. (2015) The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assay.
    J Immunol Methods. 425: 79-87.
  8. Van Der Ploeg, K. et al. (2017) Modulation of Human Leukocyte Antigen-C by Human Cytomegalovirus Stimulates KIR2DS1 Recognition by Natural Killer Cells.
    Front Immunol. 8: 298.
    9. View The Latest Product References
  9. Wijaya, R.S. et al. (2020) Expansion of dysfunctional CD56-CD16+ NK cells in chronic hepatitis B patients.
    Liver Int. Dec 23 [Epub ahead of print].

Synonyms
Killer Cell IMMUNOGLOBIN-LIKE Receptors
RRID
AB_324524
UniProt
P43626
P43627
P43628
Q14954
P43631
P43632
Entrez Gene
KIR2DL1
KIR2DL2
KIR2DL3
KIR2DS2
KIR2DS4
KIR2DS1
GO Terms
GO:0005515 protein binding
GO:0002769 natural killer cell inhibitory signaling pathway
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0050776 regulation of immune response
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity

MCA2243PE

152645

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