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IRF3 antibody | AB01/1D2

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Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of IRF3 CRISPR knockout HeLa (IRF3 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti IRF3 antibody (VMA00640) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 495 second and (B) 30 second exposure.
Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of IRF3 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti IRF3 Antibody, clone AB01/1D2 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates probed with Mouse anti Human IRF3 antibody, clone AB01/1D2 (VMA00640) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 57 second exposure. Arrow points to IRF3 (molecular weight 51 kDa).

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WB

Mouse anti IRF3

Product Type
PrecisionAb Monoclonal
Clone
AB01/1D2
Isotype
IgG2b
Format
Purified
Specificity
IRF3

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Product Code Applications Pack Size List Price Your Price Qty
VMA00640
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 100 µl loader
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loader

Mouse anti Human IRF3 antibody, clone AB01/1D2 recognizes interferon regulatory factor 3 (IRF3).

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene (provided by RefSeq, Nov 2011).

Mouse anti Human IRF3 antibody, clone AB01/1D2 detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti IRF3 antibody detects a band of approximately 51 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid.
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human IRF3 (aa 1-427)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q14653
Entrez Gene
IRF3
GO Terms
GO:0005886 plasma membrane
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003712 transcription cofactor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006355 regulation of transcription, DNA-dependent
GO:0006366 transcription from RNA polymerase II promoter
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0032480 negative regulation of type I interferon production
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway

VMA00640

170517

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