Integrin Alpha 4 antibody | Natalizumab
0.5 μg of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 μl/min; inj. volume [μl]: 3; temp.: 25 °C; detection: 280 nm; sample: mAb (1 mg/ml).
A microtiter plate was coated overnight with Human Anti-Natalizumab Antibody (clone AbD21375, HCA248) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of natalizumab (therapeutic reference product) or Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Natalizumab Antibody (clone AbD21375_hIgG1, HCA249P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Integrin Alpha 4 (Natalizumab Biosimilar)
Anti-Integrin Alpha 4 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug natalizumab (Tysabri) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.
- Product Type
- Monoclonal Antibody
- Clone
- Natalizumab
- Isotype
- IgG4
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6104 | E | 0.2 mg |
|
Human Anti-Integrin Alpha 4 Antibody is a research grade biosimilar of the monoclonal antibody drug natalizumab. It is a recombinant human IgG4 kappa antibody with variable regions from the therapeutic antibody natalizumab.
Human Anti-Integrin Alpha 4 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-natalizumab antibodies, for example HCA248 and HCA249P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-natalizumab antibody in IgG1 format as reference standard, for example HCA249.
Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn’s disease. Natalizumab is also being evaluated for potential clinical use in the treatment of B-cell malignancies. This therapeutic antibody, directed against alpha-4 integrin (CD49d), binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-natalizumab antibody products
Human Anti-Integrin Alpha 4 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-natalizumab antibodies, for example HCA248 and HCA249P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-natalizumab antibody in IgG1 format as reference standard, for example HCA249.
Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn’s disease. Natalizumab is also being evaluated for potential clinical use in the treatment of B-cell malignancies. This therapeutic antibody, directed against alpha-4 integrin (CD49d), binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-natalizumab antibody products
Product Details
- Target Species
- Human
- Product Form
- Human IgG4 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- RAMOS cell line
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
- Regulatory
- For research purposes only
Applications of Integrin Alpha 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
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