Integrin Alpha 4 antibody | Natalizumab
0.5 μg of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade) and natalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 μl/min; inj. volume [μl]: 3; temp.: 25 °C; detection: 280 nm; sample: mAb (1 mg/ml).
A microtiter plate was coated overnight with Human Anti-Natalizumab Antibody (clone AbD21375, HCA248) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of natalizumab (therapeutic reference product) or Human Anti-Integrin Alpha 4 Antibody, MCA6104 (natalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Natalizumab Antibody (clone AbD21375_hIgG1, HCA249P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Integrin Alpha 4 (Natalizumab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- Natalizumab
- Isotype
- IgG4
- Specificity
- Integrin Alpha 4
Antibody Quality and Performance Guarantee
| Human Anti-Integrin Alpha 4 Antibody is a research grade biosimilar of the monoclonal antibody drug natalizumab. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. This product is a recombinant human IgG4 kappa antibody with variable regions from the therapeutic antibody natalizumab. Human Anti-Integrin Alpha 4 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-natalizumab antibodies, for example HCA248 and HCA249P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-natalizumab antibody in IgG1 format as reference standard, for example HCA249. Natalizumab (Tysabri) is a humanized IgG4/kappa monoclonal antibody approved as a monotherapy for the treatment of relapsing forms of multiple sclerosis and for the treatment of Crohn’s disease. Natalizumab is also being evaluated for potential clinical use in the treatment of B-cell malignancies. This therapeutic antibody, directed against alpha-4 integrin (CD49d), binds to alpha-4/beta-1 integrin on the surface of circulating leucocytes. It is believed to act by blocking the interaction of alpha-4/beta-1 integrin with vascular cell adhesion molecule-1 (VCAM-1), preventing the adherence and transport of inflammatory immune cells across the intestinal lining and blood-brain barrier. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-natalizumab antibody products |
- Target Species
- Human
- Product Form
- Human IgG4 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Source
- HEK
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% ProClin 300
- Immunogen
- RAMOS cell line
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
MCA6104
151343
154399
157769
162923
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