IL-2 antibody | MQ1-17H12
Rat anti Human Interleukin-2:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- MQ1-17H12
- Isotype
- IgG2a
- Specificity
- IL-2
Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 recognizes human interleukin-2 (IL-2), also known as T-cell growth factor or Aldesleukin. IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation. Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it ascts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkins et al. 1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotze et al. 1986). Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talal et al. 1983). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | NT | 1/2 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for IL-2 antibody
-
Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
BMC Cancer. 11: 410. -
Moncrieffe, H. et al. (2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population.
J Immunol. 185 (1): 134-43.
- UniProt
- P60568
- Entrez Gene
- IL2
- GO Terms
- GO:0002903 negative regulation of B cell apoptosis
- GO:0007155 cell adhesion
- GO:0005125 cytokine activity
- GO:0005134 interleukin-2 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008083 growth factor activity
- View More GO Terms
- GO:0019209 kinase activator activity
- GO:0030101 natural killer cell activation
- GO:0030217 T cell differentiation
- GO:0030307 positive regulation of cell growth
- GO:0030890 positive regulation of B cell proliferation
- GO:0032740 positive regulation of interleukin-17 production
- GO:0034105 positive regulation of tissue remodeling
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0050729 positive regulation of inflammatory response
MCA1553A488
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