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IL-12 p40 antibody | M18

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Sandwich ELISA analysis of human IL-12 p40 using Mouse anti Human interleukin-12 p40 (MCA6194GA) as a capture reagent and biotinylated Mouse anti Human interleukin-12 p40 (MCA6195B) as a detection reagent with recombinant human IL-12 p40 as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human Interleukin-12 p40:Biotin

Product Type
Monoclonal Antibody
Clone
M18
Isotype
IgG2a
Specificity
IL-12 p40

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Product Code Applications Pack Size List Price Your Price Qty
MCA6195B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human Interleukin-12 p40 antibody, clone M18, recognizes interleukin-12 p40 (IL-12 p40) also known as interleukin-12 subunit beta and cytotoxic lymphocyte maturation factor 40 kDa subunit. IL-12 is a heterodimeric cytokine composed of 2 subunits, a 35 kDa subunit (p35) and a 40 kDa subunit (p40). The IL-12 p40 subunit is largely expressed by dendritic cells, monocytes, macrophages, neutrophils, B-lymphocytes and keratinocytes (Airoldi et al. 2002). It is able to form a homodimer, which was believed to be inactive. However it has subsequently been demonstrated to have proinflammatory properties through its induction of inducible nitric oxide synthase in microglia (Pahan et al. 2001, Jana et al. 2009). IL-12 p40 coupled to the protein p19 forms the heterodimer IL-23 (p19-p40), when this formation takes place in activated dentritic cells the p19-p40 binds to its receptor IL-12Rβ1 to activate the STAT4 signaling pathway. This results in the stimulation of IFNγ production along with proliferation of PHA blast T cells (Airoldi et al. 2002).

The biotinylated Mouse anti Human interleukin-12 p40 antibody, clone M18 (MCA6195B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-12 p40 antibody, clone D14-5G11 (MCA6194GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-12 p40 (Ile23-Ser328)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6194GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Interleukin-12 p40 MCA6194GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Interleukin-12 p40

Synonyms
IL-12B
UniProt
P29460
Entrez Gene
IL12B
GO Terms
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0007050 cell cycle arrest
GO:0050829 defense response to Gram-negative bacterium
GO:0010224 response to UV-B
GO:0010535 positive regulation of activation of JAK2 kinase activity
GO:0016477 cell migration
GO:0019953 sexual reproduction
GO:0032693 negative regulation of interleukin-10 production
GO:0032700 negative regulation of interleukin-17 production
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032729 positive regulation of interferon-gamma production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032740 positive regulation of interleukin-17 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0032819 positive regulation of natural killer cell proliferation
GO:0034105 positive regulation of tissue remodeling
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042088 T-helper 1 type immune response
GO:0042093 T-helper cell differentiation
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043382 positive regulation of memory T cell differentiation
GO:0043514 interleukin-12 complex
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0045785 positive regulation of cell adhesion
GO:0046982 protein heterodimerization activity
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0050729 positive regulation of inflammatory response
GO:0051135 positive regulation of NK T cell activation
GO:0051142 positive regulation of NK T cell proliferation
GO:0070743 interleukin-23 complex

MCA6195B

100005422

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