IFN Gamma antibody | B27

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Anti Human Interferon Gamma Antibody, clone B27 gallery image 1 — Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IFN gamma (MCA1554F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse IgG1 (MCA928F). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IFN gamma (**MCA1554F**). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human Interferon Gamma:FITC

Product Type: Monoclonal Antibody
Clone: B27
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1554F Datasheet	F* SDS	0.1 mg	Log in		Get Quote

Mouse anti Human Interferon gamma antibody, clone B27 recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.

Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favre et al. 1989).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Recombinant human IFN gamma.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee: 12 months from date of despatch

More Information

UniProt: P01579
Entrez Gene: IFNG
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0002053 positive regulation of mesenchymal cell proliferation; GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005133 interferon-gamma receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0007050 cell cycle arrest; GO:0050796 regulation of insulin secretion; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0032735 positive regulation of interleukin-12 production; GO:0032747 positive regulation of interleukin-23 production; GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0042508 tyrosine phosphorylation of Stat1 protein; GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045672 positive regulation of osteoclast differentiation; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051712 positive regulation of killing of cells of other organism; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity; GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Regulatory: For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat	1/10

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Human Seroblock	BUF070A	F	50 Test	Log in		Get Quote
Human Seroblock	BUF070B	F	200 Test	Log in		Get Quote

Application Based External Images

ELISpot

Flow Cytometry

Product Specific References

References for IFN Gamma antibody

Favre, C. et al. (1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.
Mol Immunol. 26 (1): 17-25.
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Paliard, X. et al. (1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.
J Immunol. 141 (3): 849-55.
Gomes, J.A. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
Infect Immun. 71 (3): 1185-93.
Lai, X. et al. (2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.
Clin Exp Immunol. 133: 182-92.
Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
J Immunol. 167: 3107-13.

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