Mouse anti Human Interferon gamma antibody, clone B27
recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.
Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favre et al. 1989
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Recombinant human IFN gamma.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein import into nucleus, translocation
positive regulation of mesenchymal cell proliferation
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
response to virus
interferon-gamma receptor binding
cell cycle arrest
regulation of insulin secretion
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptosis
tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of nitric oxide biosynthetic process
positive regulation of osteoclast differentiation
negative regulation of smooth muscle cell proliferation
positive regulation of membrane protein ectodomain proteolysis
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
negative regulation of metanephric nephron tubule epithelial cell differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Gamma antibody
|Flow Cytometry 1
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Negative Isotype Controls Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for IFN Gamma antibody
Favre, C. et al. (1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.
Mol Immunol. 26 (1): 17-25.
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Paliard, X. et al. (1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.
J Immunol. 141 (3): 849-55.
Gomes, J.A. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
Infect Immun. 71 (3): 1185-93.
Lai, X. et al. (2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.
Clin Exp Immunol. 133: 182-92.
Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
J Immunol. 167: 3107-13.
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