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IFN Gamma antibody | B27

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Anti Human Interferon Gamma Antibody, clone B27 gallery image 1
Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IFN gamma (MCA1554F). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse IgG1 (MCA928F). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IFN gamma (MCA1554F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Human Interferon Gamma:FITC

Product Type
Monoclonal Antibody
Clone
B27
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1554F
Datasheet
F*
MSDS
0.1 mg loader

Mouse anti Human Interferon gamma antibody, clone B27 recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.

Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favre et al. 1989).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Recombinant human IFN gamma.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01579 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0050796 regulation of insulin secretion
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032735 positive regulation of interleukin-12 production
GO:0032747 positive regulation of interleukin-23 production
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042508 tyrosine phosphorylation of Stat1 protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051712 positive regulation of killing of cells of other organism
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

ELISpot

Flow Cytometry

Product Specific References

References for IFN Gamma antibody

  1. Favre, C. et al. (1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.
    Mol Immunol. 26 (1): 17-25.
  2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  3. Paliard, X. et al. (1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.
    J Immunol. 141 (3): 849-55.
  4. Baig, J. et al. (2002) Elicitation of simian immunodeficiency virus-specific cytotoxic T lymphocytes in mucosal compartments of rhesus monkeys by systemic vaccination.
    J Virol. 76 (22): 11484-90.
  5. Gomes, J.A. et al. (2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.
    Infect Immun. 71 (3): 1185-93.
  6. Lai, X. et al. (2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.
    Clin Exp Immunol. 133: 182-92.
  7. Wakkach, A. et al. (2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.
    J Immunol. 167: 3107-13.
  8. Yamaguchi, E. et al. (1999) Differentiation of human single-positive fetal thymocytes in vitro into IL-4- and/or IFN-gamma-producing CD4+ and CD8+ T cells.
    Int Immunol. 11 (4): 593-603.
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