HSPA4 antibody | AbD23625_hIgG1
Human anti HSPA4 antibody, clone AbD23625_hIgG1 recognizes human heat shock 70 kDa protein 4, also known as epididymis secretory sperm binding protein Li 5a and APG-2. It is an 840 amino acid chaperone that protects existing proteins against aggregation as well as mediating correct folding of newly synthesized proteins.
HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting.
HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting.
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human HSPA4
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P34932
- Entrez Gene
- HSPA4
- GO Terms
- GO:0006986 response to unfolded protein
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0045040 protein import into mitochondrial outer membrane
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of HSPA4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/50 | 1/800 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Western Blotting
- Anti HSPA4 antibody detects a band of approximately 110 kDa in MCF7 cell lysate under reducing conditions
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
|
|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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