HSPA4 antibody | AbD23625_hIgG1

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Anti HSPA4 Antibody, clone AbD23625_hIgG1 gallery image 1 — Western blot analysis was performed by loading 25µl MCF7 lysate (Lanes 2-7) onto an AnykD™ Criterion™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run in lane 1. Lanes 2 & 3 and 5 & 6 were probed with Human anti HSPA4, clone AbD23625_hIgG1 (**HCA273**) at 1.25µg/ml (lanes 2 & 5) and 0.625µg/ml (lanes 3 & 6). Lanes 2-4 were probed with HRP conjugated Tidyblot™ ( STAR209P) at 1/200. Lanes 5-7 were probed with HRP conjugated Goat anti Human IgG F(ab')₂ antibody (STAR126P) at 1/2500. Visualization was carried out using the Bio-Rad Chemidoc™ Touch Imaging System

Human anti HSPA4

Product Type: Monoclonal Antibody
Clone: AbD23625_hIgG1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
HCA273	WB			0.1 mg

Summary
Useful Reagents

Human anti HSPA4 antibody, clone AbD23625_hIgG1 recognizes human heat shock 70 kDa protein 4, also known as epididymis secretory sperm binding protein Li 5a and APG-2. It is an 840 amino acid chaperone that protects existing proteins against aggregation as well as mediating correct folding of newly synthesized proteins.

HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting.

Product Details

Target Species: Human
Product Form: Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Recombinant human HSPA4
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P34932 Related reagents
Entrez Gene: HSPA4 Related reagents
GO Terms: GO:0006986 response to unfolded protein; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0034619 cellular chaperone-mediated protein complex assembly; GO:0045040 protein import into mitochondrial outer membrane
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory: For research purposes only

Applications of HSPA4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/50	1/800

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting: Anti HSPA4 antibody detects a band of approximately 110 kDa in MCF7 cell lysate under reducing conditions

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Human IgG F(ab')2:HRP	STAR126P	0.5 ml	E WB
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

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