HSPA4 antibody | AbD23625_hIgG1
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WB ResetHuman anti HSPA4
- Product Type
- Monoclonal Antibody
- Clone
- AbD23625_hIgG1
- Isotype
- IgG1
- Specificity
- HSPA4
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| Human anti HSPA4 antibody, clone AbD23625_hIgG1 recognizes human heat shock 70 kDa protein 4, also known as epididymis secretory sperm binding protein Li 5a and APG-2. It is an 840 amino acid chaperone that protects existing proteins against aggregation as well as mediating correct folding of newly synthesized proteins. HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting. |
- Target Species
- Human
- Product Form
- Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human HSPA4
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/50 | 1/800 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- Western Blotting
- Anti HSPA4 antibody detects a band of approximately 110 kDa in MCF7 cell lysate under reducing conditions.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- Synonyms
- Heat Shock 70 Kda Protein 4
- UniProt
- P34932
- Entrez Gene
- HSPA4
- GO Terms
- GO:0006986 response to unfolded protein
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0045040 protein import into mitochondrial outer membrane
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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