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HSPA4 antibody | AbD23625_hIgG1

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Anti HSPA4 Antibody, clone AbD23625_hIgG1 thumbnail image 1
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Western blot analysis was performed by loading 25μl MCF7 lysate (Lanes 2-7) onto an AnykD Criterion TGX Stain-Free gel. Precision Plus Protein™ Prestained Standards were run in lane 1. Lanes 2 & 3 and 5 & 6 were probed with Human anti HSPA4, clone AbD23625_hIgG1 (HCA273) at 1.25µg/ml (lanes 2 & 5) and 0.625µg/ml (lanes 3 & 6). Lanes 2-4 were probed with HRP conjugated Tidyblot™ ( STAR209P) at 1/200. Lanes 5-7 were probed with HRP conjugated Goat anti Human IgG F(ab')2 antibody (STAR126P) at 1/2500. Visualization was carried out using the Bio-Rad Chemidoc Touch Imaging System

Human anti HSPA4

Product Type
Monoclonal Antibody
Clone
AbD23625_hIgG1
Isotype
IgG1
Specificity
HSPA4

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Product Code Applications Pack Size List Price Your Price Qty
HCA273
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader

Human anti HSPA4 antibody, clone AbD23625_hIgG1 recognizes human heat shock 70 kDa protein 4, also known as epididymis secretory sperm binding protein Li 5a and APG-2. It is an 840 amino acid chaperone that protects existing proteins against aggregation as well as mediating correct folding of newly synthesized proteins.

HSPA4 has a predicted molecular weight of ~94 kDa; human anti HSPA4 antibody detects a band of approximately 110 kDa in western blotting.

Target Species
Human
Product Form
Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human HSPA4
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/50 1/800
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Western Blotting
Anti HSPA4 antibody detects a band of approximately 110 kDa in MCF7 cell lysate under reducing conditions.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
List Price Your Price
loader
Description Goat anti Human IgG F(ab')2:HRP
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Synonyms
Heat Shock 70 Kda Protein 4
UniProt
P34932
Entrez Gene
HSPA4
GO Terms
GO:0006986 response to unfolded protein
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0045040 protein import into mitochondrial outer membrane
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.

HCA273

1611 161580

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