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HPRT1 antibody | 5F11A7

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Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with HPRT1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 38 second exposure. Arrow points to HPRT1 (molecular weight 24 kDa).
Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of HPRT1 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti HPRT1 antibody (VMA00483) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 116 second and (B) 30 second exposure.
Anti HPRT1 Antibody, clone 5F11A7 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of HPRT1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti HPRT1

Product Type
PrecisionAb Monoclonal
Clone
5F11A7
Isotype
IgG1
Format
Purified
Specificity
HPRT1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00483
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human HPRT1 antibody recognizes HPRT1 also known as hypoxanthine-guanine phosphoribosyltransferase or HGPRTase.

The protein encoded by HPRT1 gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in HPRT1 result in Lesch-Nyhan syndrome (LNS) or gout (provided by RefSeq, Jun 2009).

Mouse anti Human HPRT1 antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti HPRT1 detects a band of approximately 24 kDa in HeLa cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human HPRT1 (aa1-218) expressed in E. Coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P00492
Entrez Gene
HPRT1
GO Terms
GO:0000287 magnesium ion binding
GO:0001975 response to amphetamine
GO:0004422 hypoxanthine phosphoribosyltransferase activity
GO:0005829 cytosol
GO:0006166 purine ribonucleoside salvage
GO:0006168 adenine salvage
GO:0006178 guanine salvage
GO:0007625 grooming behavior
GO:0019835 cytolysis
GO:0021756 striatum development
GO:0021895 cerebral cortex neuron differentiation
GO:0021954 central nervous system neuron development
GO:0032263 GMP salvage
GO:0032264 IMP salvage
GO:0042803 protein homodimerization activity
GO:0045964 positive regulation of dopamine metabolic process
GO:0046100 hypoxanthine metabolic process
GO:0046651 lymphocyte proliferation
GO:0048813 dendrite morphogenesis
GO:0051289 protein homotetramerization

VMA00483

158959 160712

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