HLA DR antibody | HL-39

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Mouse anti Human HLA DR:Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: HL-39
Isotype: IgG3
Specificity: HLA DR

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References (3)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA1879A647 Datasheet SDS SDS	F	100 Tests/1ml		Log in
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Anti Human HLA DR Antibody, clone HL-39 thumbnail image 1

Anti Human HLA DR Antibody, clone HL-39 gallery image 1 — Figure A. StarBright Blue 615 conjugated Mouse anti Human CD19 antibody, clone LT19 (**MCA1940SBB615**).
Figure B. StarBright Blue 615 conjugated Mouse anti Human CD19 antibody, clone LT19 (**MCA1940SBB615**) and Alexa Fluor^®647 conjugated Mouse anti Human HLA DR antibody, clone HL-39 (**MCA1879A647**).
All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the **ZE5 Cell analyser**.

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Mouse anti Human HLA DR antibody, clone HL-39 recognizes HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.

Mouse anti Human HLA DR antibody, clone HL-39 recognizes a determinant that is dependant upon the association of both the alpha and beta chains of HLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species: Human
Product Form: Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Raji Burkitt lymphoma cell line.
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Alexa Fluor®647 650 665
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

Source Reference

Horejsí, V. et al. (1986) Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.
Tissue Antigens. 28 (5): 288-97.

References for HLA DR antibody

Philippidis, P. et al. (2010) Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery.
Eur J Cardiothorac Surg. 37: 1360-6.
Robinson, H. et al. (2022) The effect of expressive writing on wound healing: Immunohistochemistry analysis of skin tissue two weeks after punch biopsy wounding.
J Psychosom Res. 161: 110987.