HLA B27 antibody | HLA-ABC-m3
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Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative
Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).
Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A.
- Approx. Protein Concentrations
- 50 ug/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of HLA B27 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for HLA B27 antibody
-
Trapani, J.A. et al. (1983) Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
Hum Immunol. 7 (4): 205-16. -
Orr, K. et al. (1994) Utilization of commercial antisera and flow cytometry in HLA-B27 typing.
Cytometry (Comm. Clin. Cytometry) 18: 17-20. -
Levering, W.H. et al. (2003) Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens.
Cytometry B Clin Cytom. 54: 28-38. -
Mandic, R. et al. (2004) Comparison of surface HLA class I levels in squamous cell carcinoma cell lines of the head and neck.
Anticancer Res. 24 (2B): 973-9. -
Goodall, J.C. et al. (2007) Does HLA-B27 influence the monocyte inflammatory response to lipopolysaccharide?
Rheumatology (Oxford). 46: 232-7. -
Meyer, O. et al. (2006) A simple and practical agglutination assay for human leucocyte antigen-B27 typing.
Vox Sang. 91 (1): 77-80. -
Meyer, O. et al. (2008) Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.
Tissue Antigens. 71 (2): 157-9. -
Marroquin, B. O et al. (2015) HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
PLoS One. 10 (6): e0130811.
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