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HLA B27 antibody | HLA-ABC-m3

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Mouse anti Human HLA B27:FITC

Product Type
Monoclonal Antibody
Clone
HLA-ABC-m3
Isotype
IgG2a
Specificity
HLA B27

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Product Code Applications Pack Size List Price Your Price Qty
MCA116F
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).

Flow cytometry - subjects expected fluorescence intensity:
Heterozygous HLA-B27+ = Strong.
Homozygous HLA-B27+ = Strong.
Heterozygous HLA-B7+ = Faint.
Non B27, Non B7 = Negative.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A.
Approx. Protein Concentrations
50 ug/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
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Description Mouse IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for HLA B27 antibody

  1. Trapani, J.A. et al. (1983) Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
    Hum Immunol. 7 (4): 205-16.
  2. Orr, K. et al. (1994) Utilization of commercial antisera and flow cytometry in HLA-B27 typing.
    Cytometry (Comm. Clin. Cytometry) 18: 17-20.
  3. Levering, W.H. et al. (2003) Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens.
    Cytometry B Clin Cytom. 54: 28-38.
  4. Mandic, R. et al. (2004) Comparison of surface HLA class I levels in squamous cell carcinoma cell lines of the head and neck.
    Anticancer Res. 24 (2B): 973-9.
  5. Goodall, J.C. et al. (2007) Does HLA-B27 influence the monocyte inflammatory response to lipopolysaccharide?
    Rheumatology (Oxford). 46: 232-7.
  6. Meyer, O. et al. (2006) A simple and practical agglutination assay for human leucocyte antigen-B27 typing.
    Vox Sang. 91 (1): 77-80.
  7. Meyer, O. et al. (2008) Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.
    Tissue Antigens. 71 (2): 157-9.
  8. Marroquin, B. O et al. (2015) HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
    PLoS One. 10 (6): e0130811.
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RRID
AB_322098
UniProt
P03989
Entrez Gene
HLA-B
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