HLA A2 antibody | BB7.2
Mouse anti Human HLA A2:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- BB7.2
- Isotype
- IgG2b
- Specificity
- HLA A2
Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Papain solubilized HLA-A2
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:Alexa Fluor® 488 | MCA691A488 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse IgG2b Negative Control:Alexa Fluor® 488 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock |
References for HLA A2 antibody
-
Parham, P. & Brodsky, F.M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Hum Immunol. 3 (4): 277-99. -
Hogan, K.T. & Brown, S.L. (1992) Localization and characterization of serologic epitopes on HLA-A2.
Hum Immunol. 33 (3): 185-92. -
Drukker, M. et al. (2006) Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells.
Stem Cells. 24 (2): 221-9. -
Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells.
Am J Trop Med Hyg. 82:600-7. -
Duncan, L.M. et al. (2010) Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.
J Immunol. 184: 6978-85. -
Wang, B. et al. (2004) Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
Blood. 104: 200-6. -
Wooldridge, L. et al. (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region.
Eur J Immunol. 37: 1323-33. -
Wooldridge, L. et al. (2010) MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.
J Immunol. 184: 3357-66.
View The Latest Product References
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Popovic, J. et al. (2011) The only proposed T-cell epitope derived from the TEL-AML1 translocation is not naturally processed.
Blood. 118 (4): 946-54. -
Schmidt, J. et al. (2011) Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni2+-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8+ T Cells.
J Biol Chem. 286: 41723-35. -
Brooks, S.E. et al. (2015) Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.
PLoS One. 10 (10): e0140483. -
Rothe, K. et al. (2016) Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells.
Arthritis Rheumatol. 68 (2): 337-46. -
De Angelis Rigotti, F. et al. (2017) MARCH9-mediated ubiquitination regulates MHC I export from the TGN.
Immunol Cell Biol. 95 (9): 753-64. -
Kim, J.Y. et al. (2017) Detection of Donor-Derived Microparticles in the Peripheral Blood of a Hand Transplant Recipient During Rejection.
Transplant Direct. 3 (3): e131. -
Dockree, T. et al. (2017) CD8+ T-cell specificity is compromised at a defined MHCI/CD8 affinity threshold.
Immunol Cell Biol. 95 (1): 68-76. -
Sun, W. et al. (2018) A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses.
Cell Mol Immunol. 15 (8): 768-81. -
Obenaus, M. et al. (2015) Identification of human T-cell receptors with optimal affinity to cancer antigens using antigen-negative humanized mice.
Nat Biotechnol. 33 (4): 402-7. -
Walseng, E. et al. (2015) Soluble T-cell receptors produced in human cells for targeted delivery.
PLoS One. 10 (4): e0119559.
MCA2090A488
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