Heat Shock 70 Kda Protein 5 antibody | CD01/1A7

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Mouse anti Heat Shock 70 Kda Protein 5

Heat Shock 70 Kda Protein 5 Antibody with Control Lysate

Product Type
PrecisionAb™ Monoclonal
Clone
CD01/1A7
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00570 WB 100 µl
VMA00570KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human heat shock 70 kDa protein 5 antibody recognizes heat shock 70 kDa protein 5 also known as GRP78, HSPA5 or 78 kDa glucose-regulated protein.

Heat shock 70 kDa protein 5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Mouse anti Human heat shock 70 kDa Protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human heat shock 70 kDa protein 5 (amino acids 1 - 654)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P11021 Related reagents
Entrez Gene
HSPA5 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock 70 Kda Protein 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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