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Heat Shock 70 Kda Protein 5 antibody | CD01/1A7

Mouse anti Heat Shock 70 Kda Protein 5

Product Type
PrecisionAb Monoclonal
Heat Shock 70 Kda Protein 5

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti Human heat shock 70 kDa protein 5 antibody recognizes heat shock 70 kDa protein 5 also known as GRP78, HSPA5 or 78 kDa glucose-regulated protein.

Heat shock 70 kDa protein 5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Mouse anti Human heat shock 70 kDa Protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Western Blotting
Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
E. coli-derived recombinant human heat shock 70 kDa protein 5 (amino acids 1 - 654)
For research purposes only.
12 months from date of despatch.
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Entrez Gene
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum



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