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Haptoglobin antibody | C08-1E10

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Sandwich ELISA analysis of human haptoglobin using Mouse anti Human haptoglobin (MCA6234GA) as a capture reagent and biotinylated Mouse anti Human haptoglobin (MCA6235B) as a detection reagent with purified native human haptoglobin as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human Haptoglobin:Biotin

Product Type
Monoclonal Antibody
Clone
C08-1E10
Isotype
IgG1
Specificity
Haptoglobin

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Product Code Applications Pack Size List Price Your Price Qty
MCA6235B
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Mouse anti Human haptoglobin antibody, clone C08-1E10, recognizes haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form (Redmond et al. 2018). Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues (Vanuytsel et al. 2013). A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages (Sadrzadeh & Bozorgmehr 2004). Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12 (Arredouani et al. 2005).

The biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 (MCA6235B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human haptoglobin antibody, clone E07-10E9 (MCA6234GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Native haptoglobin purified from human plasma
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6234GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P00738
Entrez Gene
HP
GO Terms
GO:0006952 defense response
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0010942 positive regulation of cell death
GO:0030492 hemoglobin binding
GO:0051354 negative regulation of oxidoreductase activity
GO:2000296 negative regulation of hydrogen peroxide catabolic process

MCA6235B

100005269

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