H3-S10-ADP-RIBOSE antibody | AbD55558

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anti H3-S10-ADP-RIBOSE

Product Type: Monoclonal Antibody
Clone: AbD55558
Isotype: Fab antibody
Specificity: H3-S10-ADP-RIBOSE

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References (3)
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Batch/Lot Specific Datasheets

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TZA0119 Datasheet SDS SDS	E WB	0.1 mg		Log in
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anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 thumbnail image 1

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 thumbnail image 2

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 thumbnail image 3

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 thumbnail image 4

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 thumbnail image 5

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 gallery image 1 — Western blot analysis of whole SDS cell extracts prepared from 2 mM H₂O₂-treated U2OS wild-type (WT) and HPF1 knock out (HPF1KO) cells, probed with anti H3-S10-ADP ribose antibody, clone AbD55558 (**TZA0119**), or the anti H3-S10-ADP ribose antibody clones AbD33644 (**TZA023**) and AbD55568. Antibodies were conjugated to HRP using BiSpyCatcher2:HRP (**TZC002P**).
Image courtesy of the Matić research group (Max Planck Institute for Biology of Ageing).

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 gallery image 2 — Western blot analysis of histone enriched sulfuric acid cell extracts prepared from 2 mM H₂O₂-treated U2OS wild-type (WT) and HPF1 knock out (HPF1KO) cells, probed with anti H3-S10-ADP ribose antibody, clone AbD55558 (**TZA0119**), or the anti H3-S10-ADP ribose antibody clones AbD33644 (**TZA023**) and AbD55568. Antibodies were conjugated to HRP using BiSpyCatcher2:HRP (**TZC002P**).
Image courtesy of the Matić research group (Max Planck Institute for Biology of Ageing).

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 gallery image 3 — **Published customer image:**
Human anti H3-S10-ADP ribose antibody, clone AbD55558 (**TZA0119**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by FLIA.
**Image caption:**
A Schematic illustration of the affinity maturation process. Illustrations generated in Adobe Illustrator. Schematics adapted from Dauben et al.20. B ELISA analysis of antibody specificities using the indicated antibodies (2 μg/mL) and biotinylated peptides (61 nM). Bars represent the arithmetic mean of 3 independent experiments. Error bars represent SD. C Left: Dot Blot analysis of isolated RNA from U2OS cells before and after glucose starvation. Right: Enzymatic digestion control of isolated RNA. Detection was done in parallel, resulting in comparable signal intensities. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 gallery image 4 — **Published customer image:**
Human anti H3-S10-ADP ribose antibody, clone AbD55558 (**TZA0119**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
G. Immunoblot analysis of SDS cell extracts from 2 mM H₂O₂-treated wild-type (WT) U2OS cells with and without 1 μM PARG inhibition treatment with the indicated antibodies. Detection was done in parallel, resulting in comparable signal intensities. Red colour represents a saturated signal. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

anti H3-S10-ADP-RIBOSE Antibody, clone AbD55558 gallery image 5 — **Published customer image:**
Human anti H3-S10-ADP ribose antibody, clone AbD55558 (**TZA0119**) conjugated to HRP via spycatcher HRP (**TZC002P**) used to detect ADP ribose by western blotting.
**Image caption:**
F. Immunoblot analysis of H2SO4 cell extracts from 2 mM H2O2-treated wild-type (WT) U2OS cells with and without 1 μM Olaparib treatment with H3S10/S28-ADPr site-specific antibodies. Detection was done in parallel, resulting in comparable signal intensities. N = 3.

From: Dauben H, Mihaljević M, Kolvenbach A, Palumbieri MD, Kapsali C, Huppertz I, Matić I.
Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
**Nat Commun. 2026 Apr 2;17(1):3216**.
doi: 10.1038/s41467-026-71311-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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WB FLA

Anti-H3-S10-ADP-ribose antibody, clone AbD55558, recognizes Histone H3, ADP-ribosylated at Serine 10. This antibody also recognizes H3-S28-ADP-ribose due to similarities between sequences flanking the two H3 target sites.

Poly(ADP-ribose) polymerase 1 (PARP1) is an early responder to DNA damage in human cells. Upon binding to genomic lesions, PARP1 is able to transfer a mono- or poly-ADP-ribose residue from nicotinamide adenine dinucleotide (NAD+) to substrate proteins, for chromatin decompaction and repair factor recruitment (Bütepage et al. 2015, Suskiewicz et al. 2020).

During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). During the DNA damage response PARP1 and HPF1 primarily target PARP1 itself and histone H3, ribosylating these targets.

This H3-S10-ADP-ribose antibody, clone AbD55558, is a high affinity H3-S10-ADP-ribose antibody that has been affinity matured from the AbD33644 parental clone (TZA023). Cell lysis in sulfuric acid is recommended to remove general cross-reactivity and enable highly sensitive detection of H3-S10-ADP-ribose by western blotting. Clone AbD55558 is tagged with a SpyTag at the C-terminus of the Fab heavy chain, enabling the user to couple to a SpyCatcher reagent for conversion to alternative formats in less than an hour.

Target Species: Human
Product Form: A monovalent human recombinant Fab selected from the HuCAL® phage display library, Expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation: Recombinant Fab antibody expressed in E. coli and purified by affinity chromatography
Source: E. coli.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.0095% MIT
Carrier Free: Yes
Immunogen: ARTKQTARKS(adpr)TGGKAPRKQLAGGK-biotin
Approx. Protein Concentrations: Total protein concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: Bio-Rad has obtained the right for the manufacture and commercialization of the antibodies from Max-Planck-Innovation GmbH, the technology transfer office of the Max Planck Institute for Biology of Ageing. Further information on the Serine ADP-ribosylation technology used to generate and characterize the antibodies can be found here: Dauber et al. 2023 and Bonfiglio et al. 2020.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for H3-S10-ADP-RIBOSE antibody

Dauben, H. et al. (2026) Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling.
Nat Commun. 17 (1): 3216.