Granzyme B antibody | GB10
Mouse anti Granzyme B:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- GB10
- Isotype
- IgG1
- Specificity
- Granzyme B
Antibody Quality and Performance Guarantee
| Mouse anti Human granzyme B antibody, clone GB10 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Purified human Granzyme B.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Biotinylated Mouse anti Human granzyme B antibody, clone GB10 may be used as a detection reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with Mouse anti Human granzyme B antibody, clone GB11 (MCA2120) used as a capture reagent.
References for Granzyme B antibody
-
Spaeny-Dekking, E.H. et al. (1998) Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo.
J Immunol. 160 (7): 3610-6. -
Rong, J. et al. (2004) Isolation and characterization of novel single-chain Fv specific for human granzyme B.
Hybrid Hybridomics. 23 (4): 219-31.
- RRID
- AB_2263754
- UniProt
- P10144
- Entrez Gene
- GZMB
- GO Terms
- GO:0005515 protein binding
- GO:0001772 immunological synapse
- GO:0004252 serine-type endopeptidase activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006922 cleavage of lamin
- GO:0019835 cytolysis
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- View More GO Terms
MCA2119B
155752
170929
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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