GM-CSF antibody | E04-5B11

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Mouse anti Human GM-CSF:Biotin

Product Type
Monoclonal Antibody
Clone
E04-5B11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6231B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human GM-CSF antibody, clone E04-5B11, recognizes Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) also known as colony stimulating factor 2. GM-CSF is a cytokine and acts as a hematopoietic grown factor and immune modulator of various immune cells. It is produced by immune cells including T cells and macrophages as well as endothelial and fibroblast cells. GM-CSF has various functions such as roles in recruiting circulating neutrophils, monocytes and lymphocytes (Shi et al. 2006). It is also involved in the maturation and activation of neutrophils and macrophages to induce microbial activity and the production of cytokines. GM-CSF elicits these functions through signaling via its receptor GM-CSFR to activate NF-kappaB or STAT5 signaling pathways (Dougan et al. 2019).

The biotinylated Mouse anti Human GM-CSF antibody, clone E04-5B11 (MCA6231B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human GM-CSF antibody, clone E01-1B6 (MCA6230GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli. derived recombinant human GM-CSF (Ala18-Glu144)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P04141
Entrez Gene
CSF2
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0032747 positive regulation of interleukin-23 production
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045740 positive regulation of DNA replication
GO:0045918 negative regulation of cytolysis
Regulatory
For research purposes only

Applications of GM-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human GM-CSF MCA6230GA E 0.1 mg loader

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